(EN) The present invention relates to a method for producing a HIV vaccine, preventing infection with HIV and/or preventing the development of HIV infection in an individual. In particular, the present invention provides formation of HIV specific antibodies capable to recognize and bind to substantially all HIV-isoforms as an immune response in an individual, which bind to existing in a current epidemiologic cohort HIV-subtypes and mutants selected after antiretroviral therapy. The present invention also relates to HIV-1 peptides/polypeptides/proteins selection with reverse panning technique, LC mass spectrometry identification of HIV-1 env peptides/polypeptides/proteins, gp120 and its fragment in particular, production of recombinant HIV-1 env peptides in suitable host with necessary glycosylation - L. tarentolae and using sterically stabilized liposomes (SSL) as an adjuvant-carrier for HIV-specific immune boost composition.
(ZH) 本发明涉及制备人免疫缺陷病毒(HIV)疫苗、预防HIV感染和/或预防HIV感染在个体中的发病方法。特别地,本发明提供了在个体中可作为免疫反应产生的HIV特异性抗体,这些抗体能够识别并结合基本上所有的HIV亚型,可结合当前流行病学中存在的HIV亚型组群和抗逆转录病毒治疗后选择出的突变体。本发明还涉及通过反向淘筛技术选择HIV-1肽/多肽/蛋白,通过液相色谱(LC)质谱联用识别HIV-1env肽/多肽/蛋白、gp120及其特定片段,在适当的具有必要的糖基化功能的宿主-利什曼原虫中重组生产HIV-1env肽,以及在HIV特异性的增强免疫反应的组合物中使用立体稳定化的脂质体(SSL)作为佐剂载体。