(EN)
The group of inventions relates to medical engineering, in particular to the immunotherapy of cancer patients. The inventive tumor vaccine based on surface tumor antigens comprises a mixture of surface tumor antigens. The method for producing a tumor vaccine consists in cultivating tumor cells and in separating surface tumor antigens. The primary culture of living tumor cells, which is pre-washed of a growth medium, is exposed to a proteases action vital for cells and the thus released surface tumor antigens are separated. The primary culture of living tumor cells is repeatedly treated by proteases at intervals necessary for recovering the surface tumor antigens by means of cells. The surface tumor antigens are accumulated until a dose thereof, required for vaccination, is obtained, the composition of the thus obtained surface tumor antigens is tested. Trypsin can be used as protease. The method for carrying out antitumor immunotherapy consists in administrating the tumor vaccine in a patient body. The inventive group of inventions makes it possible to increase the effectiveness of treatment of oncological diseases by enhancing an antitumor immune response.
(ZH)
本发明属于生物技术领域，尤其涉及抗肿瘤疫苗的制备。本发明所述制备抗肿瘤疫苗的方法，包括培养肿瘤细胞和分离表面肿瘤抗原。将预先从生长培养基上洗脱的肿瘤细胞进行培养，用蛋白酶对肿瘤细胞进行不使细胞死亡的处理，收集释放的表面抗原。在一段时间间隔后，用蛋白酶重复处理培养的活体肿瘤细胞，该时间间隔足以使得细胞表面肿瘤抗原得到恢复。积聚表面肿瘤抗原，直至达到足够的疫苗剂量，控制表面肿瘤抗原的组成。蛋白酶可以采用胰蛋白酶。
