Processing

Please wait...

Settings

Settings

Goto Application

1. CN101368206 - Sequencing reaction small chamber, gene sequencing reaction bench and gene sequencing system

Office China
Application Number 200810132008.8
Application Date 16.07.2008
Publication Number 101368206
Publication Date 18.02.2009
Grant Number 101368206
Grant Date 22.08.2012
Publication Kind B
IPC
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
B01L 3/5027
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
B01L 7/52
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
7Heating or cooling apparatus
52with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
B01L 2200/0684
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2200Solutions for specific problems relating to chemical or physical laboratory apparatus
06Fluid handling related problems
0684Venting, avoiding backpressure, avoid gas bubbles
B01L 2300/0636
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
06Auxiliary integrated devices, integrated components
0627Sensor or part of a sensor is integrated
0636Integrated biosensor, microarrays
B01L 2300/0822
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0809rectangular shaped
0822Slides
B01L 2300/0877
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
08Geometry, shape and general structure
0861Configuration of multiple channels and/or chambers in a single devices
0877Flow chambers
Applicants Shenzhen HYK Gene Technology Co., Ltd.
深圳华因康基因科技有限公司
Inventors Sheng Sitong
盛司潼
Agents hu haiguo wang yanchun
Title
(EN) Sequencing reaction small chamber, gene sequencing reaction bench and gene sequencing system
(ZH) 测序反应小室、基因测序反应台及基因测序设备
Abstract
(EN)
The invention provides a sequencing reaction cabinet, used for carrying out the sequencing reaction between DNA fragments and a reagent; the sequencing reaction cabinet comprises a reaction cavity, on the inner wall at one side of which is fixedly provided with a plurality of DNA fragments; a reagent inlet and a reagent outlet which are respectively arranged at the two ends of the inner wall at the other side of the reaction cavity and are respectively used for the reagent to flow into and out of the reaction cavity. In the sequencing reaction cabinet, a plurality of DNA fragments are fixed in the reaction cavity with a small capacity as a short label array for sequencing to lead the sequencing reaction to be carried out in the reagent without a diffusion barrier, thus greatly improving the accessibility between the reagent and the DNA fragments and further shortening the reaction time; the requirements to the concentration and the dosage of the reagent is lower, thus consuming less reagent and reducing the sequencing cost. While a plurality of DNA fragments are simultaneously fixed in the reaction cabinet to provide a platform for realizing the parallel reaction of a plurality of fragments. The sequencing reaction cabinet used for carrying out sequencing reaction between DNA fragments and a reagent realizes the automatization of high pass sequencing and can realizes fast sequencing reaction.

(ZH)

本发明提出一种测序反应小室,用于DNA片段与试剂进行测序反应,包括:反应腔,所述反应腔的一侧内壁上固定多个DNA片段;试剂入口和试剂出口,分别设于反应腔另一侧内壁的两端,分别用于供试剂流入反应腔中和供试剂从反应腔中流出。本发明将多个DNA片段固定在小容量反应腔中以作为测序时的短标签阵列,使得测序反应在没有扩散势垒的试剂中进行,大大提高试剂与DNA片段的可接触性,进而缩短反应时间;对试剂的浓度和剂量要求较低,即对试剂的消耗较少,降低测序成本。而多个DNA片段的同时固定在一个反应小室里为对于多个片断的平行反应的实现提供一个平台。本发明实现高通量测序的自动化,可实现快速测序反应。

Also published as