The invention provides a sequencing reaction cabinet, used for carrying out the sequencing reaction between DNA fragments and a reagent; the sequencing reaction cabinet comprises a reaction cavity, on the inner wall at one side of which is fixedly provided with a plurality of DNA fragments; a reagent inlet and a reagent outlet which are respectively arranged at the two ends of the inner wall at the other side of the reaction cavity and are respectively used for the reagent to flow into and out of the reaction cavity. In the sequencing reaction cabinet, a plurality of DNA fragments are fixed in the reaction cavity with a small capacity as a short label array for sequencing to lead the sequencing reaction to be carried out in the reagent without a diffusion barrier, thus greatly improving the accessibility between the reagent and the DNA fragments and further shortening the reaction time; the requirements to the concentration and the dosage of the reagent is lower, thus consuming less reagent and reducing the sequencing cost. While a plurality of DNA fragments are simultaneously fixed in the reaction cabinet to provide a platform for realizing the parallel reaction of a plurality of fragments. The sequencing reaction cabinet used for carrying out sequencing reaction between DNA fragments and a reagent realizes the automatization of high pass sequencing and can realizes fast sequencing reaction.

本发明提出一种测序反应小室，用于DNA片段与试剂进行测序反应，包括：反应腔，所述反应腔的一侧内壁上固定多个DNA片段；试剂入口和试剂出口，分别设于反应腔另一侧内壁的两端，分别用于供试剂流入反应腔中和供试剂从反应腔中流出。本发明将多个DNA片段固定在小容量反应腔中以作为测序时的短标签阵列，使得测序反应在没有扩散势垒的试剂中进行，大大提高试剂与DNA片段的可接触性，进而缩短反应时间；对试剂的浓度和剂量要求较低，即对试剂的消耗较少，降低测序成本。而多个DNA片段的同时固定在一个反应小室里为对于多个片断的平行反应的实现提供一个平台。本发明实现高通量测序的自动化，可实现快速测序反应。