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1. (CN101287840) Cell lines for expressing enzyme useful in the preparation of amidated products

Office : China
Application Number: 200680030902.3 Application Date: 23.06.2006
Publication Number: 101287840 Publication Date: 15.10.2008
Grant Number: 101287840 Grant Date: 10.04.2013
Publication Kind : B
Prior PCT appl.: Application Number:PCTUS2006024687 ; Publication Number:2007002532 Click to see the data
IPC:
C12P 21/04
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
04
Cyclic or bridged peptides or polypeptides, e.g. bacitracin
CPC:
C12P 21/00
C12N 9/0071
Applicants: Unigene Lab Inc.
尤尼基因实验室公司
Inventors: Miller Duncan A.
D·A·米勒
Mehta Nozer M.
N·M·梅塔
Consalvo Angelo P.
A·P·康萨尔沃
Agents: chengmiao liuyue
中国专利代理(香港)有限公司 72001
中国专利代理(香港)有限公司 72001
Priority Data: 60/693,612 24.06.2005 US
Title: (EN) Cell lines for expressing enzyme useful in the preparation of amidated products
(ZH) 表达在酰胺化产物的制备中有用的酶的细胞系
Abstract: front page image
(EN) Cell lines are provided for expressing peptidylglycine alpha-amidating monooxygenase (PAM), or one of its two catalytic domains. High levels of enzyme expression are achieved while utilizing a non-animal source, low protein tissue culture medium. A robust two-step downstream purification results in high enzyme purity. Resulting PAM, or its PHM catalytic domain, is used to catalyze the enzymatic conversion of X-Gly to X-alpha-hydroxy-Gly or X-NH2 (X being a peptide or any chemical compound having a carbonyl group to which a glycine group can be covalently attached). Methods of preparing preferred cell lines are also set forth.
(ZH)

提供了表达肽基甘氨酸α-酰胺化单加氧酶(PAM)或它的两个催化结构域之一的细胞系。在利用非动物来源的、低蛋白组织培养基时实现了高水平酶表达。强大的两步下游纯化产生了高的酶纯度。产生的PAM或它的PHM催化结构域被用于催化X-Gly向X-α-羟基-Gly或X-NH2(X是肽或任何化合物,其具有可以共价连接甘氨酸基团的羰基)的转化。还阐述了制备优选的细胞系的方法。


Also published as:
EP1907561JP2008543344JP2011139714JP2012016354CA2613471ES2437068
AU2006261909DK1907561WO/2007/002532