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1. (CN101248183) Nucleotide sequences mediating plant male fertility and method of using same

Office : China
Application Number: 200680030863.7 Application Date: 22.06.2006
Publication Number: 101248183 Publication Date: 20.08.2008
Publication Kind : A
Prior PCT appl.: Application Number:PCTUS2006024273 ; Publication Number:2007002267 Click to see the data
IPC:
C12N 15/82
C12N 15/29
C12N 15/55
C12N 15/10
C12N 15/31
A01H 1/02
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
29
Genes encoding plant proteins, e.g. thaumatin
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
52
Genes encoding for enzymes or proenzymes
55
Hydrolases (3)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
31
Genes encoding microbial proteins, e.g. enterotoxins
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1
Processes for modifying genotypes
02
Methods or apparatus for hybridisation; Artificial pollination
CPC:
C12N 15/8231
C07K 14/415
C12N 15/8287
C12N 15/8289
Applicants: Pioneer Hi Bred Int
先锋高级育种国际公司
Inventors: Wu Yongzhong
M·C·阿尔伯特森
Albertsen Marc C.
T·W·福克斯
Trimnell Mary
H·P·赫尔希
Fox Timothy W.
G·A·哈夫曼
Hershey Howard P.
M·特林内尔
Huffman Gary A.
Y·吴
Agents: chengmiao liuyue
中国专利代理(香港)有限公司
中国专利代理(香港)有限公司
Priority Data: 11166609 24.06.2005 US
Title: (EN) Nucleotide sequences mediating plant male fertility and method of using same
(ZH) 介导植物雄性生育力的核苷酸序列以及使用其的方法
Abstract: front page image
(EN) Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants. In one such method, the homozygous recessive condition of male sterility causing alleles is maintained after crossing with a second plant, where the second plant contains a restoring transgene construct having a nucleotide sequence which reverses the homozygous condition. The restoring sequence is linked with a hemizygous sequence encoding a product inhibiting formation or function of male gametes. The maintainer plant produces only viable male gametes which do not contain the restoring transgene construct. Increase of the maintainer plant is also provided by self-fertilization, and selection for seed or plants which contain the construct.
(ZH)

本发明描述了在植株中介导雄性生育力的核苷酸序列以及示出的DNA分子和氨基酸序列。本发明还鉴定了启动子序列及其必要区域。所述核苷酸序列可用于在植株中介导雄性生育力。在一种此类方法中,在与第二植株杂交后,导致雄性不育的等位基因的纯合隐性状态被保持,其中,所述第二植株含有恢复性的转基因构建体,所述构建体具有能逆转纯合状态的核苷酸序列。所述恢复性序列与编码抑制雄性配子功能或形成的产物的半合序列相连。保持系植株仅产生不含恢复性转基因构建体的可育雄性配子。还通过自体授精以及选择含有所述构建体的种子或植物来提供保持系植株的增加。


Also published as:
MXMX/a/2008/000035EP2278019EP1899469EP2295585BRPI0613133-6JP2008543341
CA2613336AU2006262185VN25200VN18756ID048.4254IN10081/DELNP/2007
WO/2007/002267