Processing

Please wait...

Settings

Settings

Goto Application

1. CN101120089 - Method for separating and purifying nucleic acid

Office China
Application Number 200680003744.2
Application Date 02.03.2006
Publication Number 101120089
Publication Date 06.02.2008
Publication Kind A
IPC
C12N 15/09
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
CPC
C12N 15/1006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
Applicants Fuji Photo Film Co., Ltd.
富士胶片株式会社
Inventors Takeshita Yumiko
竹下由美子
Komazawa Hiroyuki
驹泽宏幸
Watanabe Shin-ichi
渡边信一
Agents dingye beng zhangtian shu
北京天昊联合知识产权代理有限公司
北京天昊联合知识产权代理有限公司
Priority Data 2005060407 04.03.2005 JP
Title
(EN) Method for separating and purifying nucleic acid
(ZH) 分离和纯化核酸的方法
Abstract
(EN)
A method for separating and purifying nucleic acid, the method comprising: (1) a step of contacting a sample solution containing nucleic acid with a solid phase to adsorb the nucleic acid on the solid phase; (2) a step of contacting a washing solution with the solid phase to wash the solid phase in a state that the nucleic acid is adsorbed on the solid phase; and (3) a step of contacting a recovering solution with the solid phase to desorb the nucleic acid from the solid phase, wherein the sample solution is prepared by including a step of removing a precipitate component, and adding a surfactant and a water-soluble organic solvent to a supernatant solution of the precipitate.

(ZH)

一种分离和纯化核酸的方法,该方法包括:(1)将含有核酸的样品溶液与固相接触,从而使核酸吸附到所述固相上的步骤;(2)将洗涤液与所述固相接触,从而在所述核酸被吸附在所述固相上的状态下洗涤该固相的步骤;以及(3)将回收液与所述固相接触,从而使核酸从所述固相上解吸附的步骤,其中,所述样品溶液是通过包括除去沉淀成分并将表面活性剂和水溶性有机溶剂加入该沉淀的上清液中的步骤的方法制得的。