(EN)
A total internal reflection fluorescent (TIRF) microscope has a conjugate lens (30) which is positioned off of the primary microscope axis (AA). The lens (30) is held by a mount (60) allowing the radial spacing between the microscope axis (AA) and the conjugate lens axis (BB) to be varied. The mount (60) also allows the lens to be rotated around the microscope axis. As the lens (30) rotates, the spot of light (64) created by the incident laser beam moves around the periphery of the objective lens (34). This changes the direction of polarisation of the evanescent wave within which the sample (44) sits. By studying the emitted fluorescence as a function of lens angle, information is provided about the spatial attitude of a molecule under study.
(ZH) 一种全内反射荧光(TIRF)显微镜具有共轭透镜30，其被放置在偏离主显微镜轴AA的位置。透镜30被载片固定，以使显微镜轴AA和共轭透镜轴BB之间的径向间距可被改变。载片60还使透镜绕显微镜轴旋转。当透镜30旋转时，由入射激光束产生的光斑64绕物镜34的圆周移动。这改变了隐失波的极化方向，样本44位于隐失波中。通过将发射的荧光作为透镜角的函数进行研究，可提供有关研究中的分子的空间姿态的信息。
