(EN)
The present invention refers to methods and devices for the detection of polymorphisms in a nucleic acid sample (e.g. blood, sperm, saliva, cells,...). To enhance the efficiency and the reliability of the known methods (e.g. DGGE, SSCP and TGGE) the amplification process (e.g. PCR) preceding the actual detection step of performed in or on the polyacrylamide gel. Multiple gradients (of chemical denaturants, thermal denaturants and of porosity of the gel matrix) are used for the separation of DNA fragments, by zone electrophoresis on gel slabs or by capillary electrophoresis.
(ZH) 本发明涉及检测核酸样品(例如血液、精液、唾液、细胞…)多态性的方法和装置。为提高已知方法(例如DGGE、SSCP和TGGE)的效率和可靠性,实际检测步骤以前在聚丙烯酰胺凝胶之中或其上进行扩增过程(例如PCR)。通过凝胶平板区带电泳或毛细管电泳,使用(化学变性剂、热变性剂以及多孔性凝胶基质)多种梯度分离DNA片段。