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1. CN1270631 - Process for producing purine nucleosides via fermentation

Office
China
Application Number 98809054.6
Application Date 17.07.1998
Publication Number 1270631
Publication Date 18.10.2000
Grant Number 1187446
Grant Date 02.02.2005
Publication Kind C
IPC
C12N 1/21
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 15/09
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
//(C12N1/21,C12R1∶19)(C12P19/32,C12R1∶19)
CPC
C12N 9/1077
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
1048Glycosyltransferases (2.4)
1077Pentosyltransferases (2.4.2)
C12P 19/38
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
38Nucleosides
C12P 19/40
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
38Nucleosides
40having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
C12N 1/205
C12R 2001/19
C12N 1/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
20Bacteria
Applicants Ajinomoto Co., Inc.
味之素株式会社
Inventors Matsui Hiroshi
松井裕
Kawasaki Hisashi
川崎寿
Shimaoka Megumi
岛冈惠
竹中康浩
仓桥修
Agents tan mengsheng
中国专利代理(香港)有限公司
Priority Data 194603/1997 18.07.1997 JP
Title
(EN) Process for producing purine nucleosides via fermentation
(ZH) 通过发酵生产嘌呤核苷的方法及所用的微生物
Abstract
(EN) A process for producing purine nucleosides which comprises breeding and culturing microorganisms having a gene encoding an enzyme wherein the feedback inhibition has been substantially relieved by substituting one or two amino acid residues in PRPP amidotransferase encoded by Escherichia coli purF, a gene encoding a protein wherein the repressor in the purine nucleotide biosynthesis system encoded by purR has been inactivated and a gene encoding an enzyme wherein purine nucleoside phosphorylase encoded by deoD has been inactivated, a gene encoding an enzyme wherein succinyl-AMP synthase encoded by purA has been inactivated, a gene encoding an enzyme wherein 6-phosphogluconate dehydratase encoded by edd has been inactivated and a gene encoding an enzyme wherein 6-phosphoglucose isomerase encoded by pgi has been inactivated, or the like.
(ZH)

生产嘌呤核苷的方法,包括培育并培养以下微生物:具有编码一种酶的基因的微生物,其中由于大肠杆菌的purF编码的PRPP转酰胺酶中一个或两个氨基酸被取代,所述反馈抑制被大致解除;具有编码一种蛋白的基因的微生物,其中嘌呤核苷生物合成系统中purR编码的阻抑物被失活;具有编码一种酶的基因的微生物,其中deoD编码的嘌呤核苷磷酸化酶被失活;具有编码一种酶的基因的微生物,其中purA编码的琥珀酰-AMP合酶被失活;具有编码一种酶的基因的微生物,其中edd编码的6-磷酸葡糖酸脱水酶被失活;以及具有编码一种酶的基因的微生物,其中pgi编码的6-磷酸葡萄糖异构酶被失活等。