(EN) This document relates to materials and methods for identifying and/or quantifying immunoglobulins from a biological sample without pre-purification of the immunoglobulins prior to ionization and detection using mass spectrometry. For example, a monoclonal light chain from a monoclonal immunoglobulin may be observed using matrix assisted laser desorption ionization - time of flight (MALDI-TOF) massspectrometry after diluting a sample containing the monoclonal immunoglobulin with an aqueous buffer containing acid and a reducing agent then mixing the sample with alpha- cyano-4-hydroxycinnamic acid matrix (CHCA). In another example, an intact monoclonal immunoglobulin may be observed in a sample using MALDI-TOF mass spectrometry after diluting the sample containing the monoclonal immunoglobulin with water then mixing the sample with CHCA matrix.
(ZH) 本文件涉及用于鉴定和/或定量来自生物样品的免疫球蛋白的材料和方法,其中在使用质谱进行电离和检测之前无需对免疫球蛋白进行预纯化。例如,在用含有酸和还原剂的水性缓冲液稀释含有单克隆免疫球蛋白的样品,然后将所述样品与α‑氰基‑4‑羟基肉桂酸基质(CHCA)混合之后,可以使用基质辅助激光解吸电离飞行时间(MALDI‑TOF)质谱观察来自单克隆免疫球蛋白的单克隆轻链。在另一个实例中,在用水稀释含有单克隆免疫球蛋白的样品,然后将所述样品与CHCA基质混合后,可以使用MALDI‑TOF质谱在样品中观察到完整的单克隆免疫球蛋白。