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1. CN112136046 - IDENTIFICATION OF IMMUNOGLOBULINS USING MASS SPECTROMETRY

Office
China
Application Number 201980030264.2
Application Date 03.05.2019
Publication Number 112136046
Publication Date 25.12.2020
Publication Kind A
IPC
G01N 33/574
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
G01N 33/68
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
G01N 33/57426
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
57407Specifically defined cancers
57426leukemia
G01N 33/6851
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6848Methods of protein analysis involving mass spectrometry
6851Methods of protein analysis involving laser desorption ionisation mass spectrometry
G01N 33/6854
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6854Immunoglobulins
G01N 2800/52
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2800Detection or diagnosis of diseases
52Predicting or monitoring the response to treatment; Prognosis
G01N 30/7266
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography ; or field flow fractionation;
02Column chromatography
62Detectors specially adapted therefor
72Mass spectrometers
7233interfaced to liquid or supercritical fluid chromatograph
724Nebulising, aerosol formation or ionisation
7266by electric field, e.g. electrospray
Applicants THE BINDING SITE GROUP LIMITED
结合点集团有限公司
Inventors SAKRIKAR DHANANJAY
达纳杰·萨克里卡
HARDING STEPHEN
斯蒂芬·哈丁
BARNIDGE DAVID
大卫·巴尼奇
LAJKO MICHELLE
米歇尔·拉杰科
Agents 北京英赛嘉华知识产权代理有限责任公司 11204
北京英赛嘉华知识产权代理有限责任公司 11204
Priority Data 1808529.0 24.05.2018 GB
62/667,076 04.05.2018 US
Title
(EN) IDENTIFICATION OF IMMUNOGLOBULINS USING MASS SPECTROMETRY
(ZH) 使用质谱鉴定免疫球蛋白
Abstract
(EN) This document relates to materials and methods for identifying and/or quantifying immunoglobulins from a biological sample without pre-purification of the immunoglobulins prior to ionization and detection using mass spectrometry. For example, a monoclonal light chain from a monoclonal immunoglobulin may be observed using matrix assisted laser desorption ionization - time of flight (MALDI-TOF) massspectrometry after diluting a sample containing the monoclonal immunoglobulin with an aqueous buffer containing acid and a reducing agent then mixing the sample with alpha- cyano-4-hydroxycinnamic acid matrix (CHCA). In another example, an intact monoclonal immunoglobulin may be observed in a sample using MALDI-TOF mass spectrometry after diluting the sample containing the monoclonal immunoglobulin with water then mixing the sample with CHCA matrix.
(ZH) 本文件涉及用于鉴定和/或定量来自生物样品的免疫球蛋白的材料和方法,其中在使用质谱进行电离和检测之前无需对免疫球蛋白进行预纯化。例如,在用含有酸和还原剂的水性缓冲液稀释含有单克隆免疫球蛋白的样品,然后将所述样品与α‑氰基‑4‑羟基肉桂酸基质(CHCA)混合之后,可以使用基质辅助激光解吸电离飞行时间(MALDI‑TOF)质谱观察来自单克隆免疫球蛋白的单克隆轻链。在另一个实例中,在用水稀释含有单克隆免疫球蛋白的样品,然后将所述样品与CHCA基质混合后,可以使用MALDI‑TOF质谱在样品中观察到完整的单克隆免疫球蛋白。