(EN) The invention provides an enzyme reaction solution for constructing a sequencing library and an application of the enzyme reaction solution. The enzyme reaction solution comprises an enzyme composition and a reaction buffer solution, wherein the enzyme composition comprises endonuclease, DNA polymerase and polynucleotide kinase; and the reaction buffer solution comprises a metal salt, a substrateand a buffer medium aqueous solution. By optimizing the formula of the enzyme reaction solution and realizing cutting, tail end repairing and A tail adding treatment on a nucleic acid sample through aone-step reaction, in a proper buffer system, the enzyme digestion reaction rate and the tail end repairing reaction rate are balanced; and under the conditions that the initial amount of the sampleis 100 pg to 1 mu g and the treatment time is the same, a sequencing library with consistent length distribution is obtained.
(ZH) 本发明提供了一种用于构建测序文库的酶反应液及其应用,所述酶反应液包括酶组合物和反应缓冲液;所述酶组合物包括核酸内切酶、DNA聚合酶和多聚核苷酸激酶;所述反应缓冲液包括金属盐、底物和缓冲介质水溶液。本发明通过优化酶反应液的配方,通过一步反应实现对核酸样本的切割、末端修复和加A尾处理,在适宜的缓冲体系中,酶切反应速率和末端修复反应速率达到平衡,在样本起始量为100pg‑1μg、处理时间相同的情况下,获得了长度分布一致的测序文库。