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1. CN111638345 - Kit and method for detecting E-Cadherin gene mutation of peripheral blood circulating tumor cells of non-small cell lung cancer patient

Office
China
Application Number 202010626836.8
Application Date 01.07.2020
Publication Number 111638345
Publication Date 08.09.2020
Publication Kind A
IPC
G01N 33/577
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
577involving monoclonal antibodies
G01N 33/574
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
G01N 33/569
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
B01D 29/05
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
29Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/-B01D27/170;   Filtering elements therefor
01with flat filtering elements
05supported
CPC
G01N 33/577
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
577involving monoclonal antibodies ; binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens;
G01N 33/57423
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
57407Specifically defined cancers
57423of lung
G01N 33/57492
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
574for cancer
57484involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
57492involving compounds localized on the membrane of tumor or cancer cells
G01N 33/56966
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
56966Animal cells
B01D 29/05
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
29Other filters with filtering elements stationary during filtration, e.g. pressure or suction filters, or filtering elements therefor
01with flat filtering elements
05supported
G01N 2333/705
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
435from animals; from humans
705Assays involving receptors, cell surface antigens or cell surface determinants
Applicants SHANDONG KAIGE INTELLIGENT MACHINE CO., LTD.
山东凯歌智能机器有限公司
Inventors LI CHENCHEN
李琛琛
HONG YING
洪瑛
HOU JINGYANG
侯景阳
LI WENTAO
李文涛
Title
(EN) Kit and method for detecting E-Cadherin gene mutation of peripheral blood circulating tumor cells of non-small cell lung cancer patient
(ZH) 检测非小细胞肺癌患者外周血循环肿瘤细胞E-Cadherin基因突变的试剂盒及方法
Abstract
(EN) The invention provides a kit and method for detecting E-Cadherin gene mutation of peripheral blood circulating tumor cells of a non-small cell lung cancer patient, and belongs to the technical field of molecular biology. The kit comprises a diluent, a decolorizing solution, a staining solution A, a staining solution B, a mouse anti-human E-Cadherin monoclonal antibody, goat anti-mouse IgG/HRP, SABC, 0.1% Triton X-100, 0.3% H2O2, a reagent A, a reagent B and a 6 * PBS buffer solution. According to the detection method provided by the invention, the E-Cadherin expression condition of the patientsuffering from advanced or recurrent non-small cell lung cancer can be detected without obtaining a tissue specimen through needle biopsy. The technology belongs to minimally invasive technology andcan realize real-time detection. The false positive result caused by the edge effect possibly generated in the dyeing process can be avoided, the stability is high, the cell loss is reduced, and the detection accuracy is improved.
(ZH) 本发明提供了检测非小细胞肺癌患者外周血循环肿瘤细胞E‑Cadherin基因突变的试剂盒及方法,属于分子生物学技术领域。该试剂盒包括稀释液、脱色液、染色液A、染色液B、鼠抗人E‑Cadherin单克隆抗体、山羊抗小鼠IgG/HRP、SABC、0.1%Triton X‑100、0.3%H2O2、试剂A、试剂B、6×PBS缓冲液。本发明提供的检测方法,不用穿刺活检获取组织标本即可检测到晚期或复发非小细胞肺癌患者E‑Cadherin表达情况。该技术属于微创,并能够实时检测。能够避免染色过程中可能产生的边缘效应导致的假阳性结果,稳定性好,降低细胞的损失,提高检测的准确性。
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