(EN) The invention provides a kit and method for detecting E-Cadherin gene mutation of peripheral blood circulating tumor cells of a non-small cell lung cancer patient, and belongs to the technical field of molecular biology. The kit comprises a diluent, a decolorizing solution, a staining solution A, a staining solution B, a mouse anti-human E-Cadherin monoclonal antibody, goat anti-mouse IgG/HRP, SABC, 0.1% Triton X-100, 0.3% H2O2, a reagent A, a reagent B and a 6 * PBS buffer solution. According to the detection method provided by the invention, the E-Cadherin expression condition of the patientsuffering from advanced or recurrent non-small cell lung cancer can be detected without obtaining a tissue specimen through needle biopsy. The technology belongs to minimally invasive technology andcan realize real-time detection. The false positive result caused by the edge effect possibly generated in the dyeing process can be avoided, the stability is high, the cell loss is reduced, and the detection accuracy is improved.
(ZH) 本发明提供了检测非小细胞肺癌患者外周血循环肿瘤细胞E‑Cadherin基因突变的试剂盒及方法,属于分子生物学技术领域。该试剂盒包括稀释液、脱色液、染色液A、染色液B、鼠抗人E‑Cadherin单克隆抗体、山羊抗小鼠IgG/HRP、SABC、0.1%Triton X‑100、0.3%H2O2、试剂A、试剂B、6×PBS缓冲液。本发明提供的检测方法,不用穿刺活检获取组织标本即可检测到晚期或复发非小细胞肺癌患者E‑Cadherin表达情况。该技术属于微创,并能够实时检测。能够避免染色过程中可能产生的边缘效应导致的假阳性结果,稳定性好,降低细胞的损失,提高检测的准确性。