Processing

Please wait...

Settings

Settings

Goto Application

1. CN109724949 - Preparation method of up-conversion luminescence flexible hybrid membrane for visual detection of tumor markers

Office China
Application Number 201910226383.7
Application Date 25.03.2019
Publication Number 109724949
Publication Date 07.05.2019
Grant Number 109724949
Grant Date 23.07.2019
Publication Kind B
IPC
G01N 21/63
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
G01N 21/359
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
35using infra-red light
359using near infra-red light
CPC
G01N 21/359
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
35using infra-red light
359using near infra-red light
G01N 21/63
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
G01N 21/64
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G01N 33/48
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
Applicants QINGDAO UNIVERSITY
青岛大学
Inventors JIN HUI
金辉
GUI RIJUN
桂日军
SUN YUJIAO
孙玉娇
JIANG XIAOWEN
姜晓文
Agents 青岛高晓专利事务所(普通合伙) 37104
Title
(EN) Preparation method of up-conversion luminescence flexible hybrid membrane for visual detection of tumor markers
(ZH) 一种用于肿瘤标志物可视化检测的柔性杂化膜的制备方法
Abstract
(EN)
The invention belongs to the technical field of preparation of flexible hybrid membrane materials and visual detection probes, and particularly relates to a preparation method of an up-conversion luminescence flexible hybrid membrane for visual detection of tumor markers. Metal ion doped black phosphorus quantum dots M-BPQDs are prepared by adopting ultrasonic and solvothermal processes; mesoporous SiO2 grows on the surfaces of the M-BPQDs and amination modification is performed; the M-BPQDs are connected with carboxylated single-stranded DNA1; receptor molecules enter holes; single-stranded DNA2 aptamers and the DNA1 are combined due to base complementation to package receptors in the holes; and an M-BPQDs probe is prepared. DNA1 terminal-SH and a composite membrane formed by assembling polymethyl methacrylate-polyimide-gold nanoparticles in a layer-by-layer manner are combined b Au-S bonds, and the membrane and the probe are connected to construct the flexible hybrid membrane. Compared with the prior art, the flexible hybrid membrane is simple to prepare, low in cost and high in sensitivity; and when a biological fluid sample containing a tumor marker is dropwise added to the hybrid membrane, a relation between the up-conversion luminescence intensity and the concentration of the marker is constructed under the excitation of near-infrared light, so that the visual detection of the marker is realized.

(ZH)
本发明属于柔性杂化膜材料和可视化检测探针的制备技术领域,具体涉及一种用于肿瘤标志物可视化检测的上转换发光柔性杂化膜的制备方法。采用超声和溶剂热制备金属离子掺杂黑磷量子点M‑BPQDs,在其表面生长介孔SiO2和氨基化改性,连接羧基化单链DNA1,受体分子进入孔内,单链DNA2适体与DNA1因碱基互补结合封装受体于孔内,制得M‑BPQDs探针。DNA1终端‑SH与聚异丁烯酸甲酯‑聚酰亚胺‑金纳米粒层层组装复合薄膜以Au‑S键结合,连接薄膜与探针构筑柔性杂化膜。与现有技术相比,本发明柔性杂化膜的制备简单,成本低,灵敏度高,当含有肿瘤标志物的生物流体样品滴加到杂化膜上,在近红外光激发下构建上转换发光强度与标志物浓度之间的联系,实现标志物可视化检测。

Also published as