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1. CN109536581 - Method for detecting embryo health by using blastocyst culture solution, and product

Office China
Application Number 201811647225.0
Application Date 29.12.2018
Publication Number 109536581
Publication Date 29.03.2019
Publication Kind A
IPC
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
C12M 1/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
34Measuring or testing with condition measuring or sensing means, e.g. colony counters
CPC
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Applicants XUKANG MEDICAL TECHNOLOGY (SUZHOU) CO., LTD.
序康医疗科技(苏州)有限公司
Inventors YAO YAXIN
姚雅馨
LI WENLU
李文璐
MA JIELIANG
马杰良
LU SIJIA
陆思嘉
Agents 上海一平知识产权代理有限公司 31266
上海一平知识产权代理有限公司 31266
Title
(EN) Method for detecting embryo health by using blastocyst culture solution, and product
(ZH) 一种利用囊胚培养液检测胚胎健康状况的方法和产品
Abstract
(EN)
The invention provides a method for detecting embryo health by using a blastocyst culture solution, and a product. In particular, the invention provides the method for detecting embryo health by usinga blastocyst culture solution in vitro. The method is characterized by comprising the following steps: (a) providing a first blastocyst culture system, wherein the first blastocyst culture system contains blastocysts with culture days of D5-D6 in vitro; (b) transferring the blastocysts to a second blastocyst culture system containing a fresh blastocyst culture solution for liquid exchange cultureso as to obtain a cultured blastocyst culture solution, wherein the liquid exchange culture time is T1; (c) taking out the cultured blastocyst culture solution to obtain a cell-free cultured blastocyst culture solution, namely a detection sample; and (d) performing gene detection on the detection sample to identify the health condition of the blastocysts. The method provided by the invention canremove the risk of pollution interference of superfluous sperms and maternal granulosa cells in the IVF and ICSI technical processes, and can accurately identify the health condition of embryos.

(ZH)
本发明提供了一种利用囊胚培养液检测胚胎健康状况的方法和产品。具体地,本发明提供了一种体外利用囊胚培养液检测胚胎健康状况的方法,其特征在于,包括步骤:(a)提供第一囊胚培养体系,所述第一囊胚培养体系含有体外培养天数为D5‑D6的囊胚;(b)将所述囊胚转移到含新鲜囊胚培养液的第二囊胚培养体系中进行换液培养,所述换液培养的时间为T1,从而获得经培养的囊胚培养液;(c)将所述经培养的囊胚培养液取出,从而获得无细胞的经培养的囊胚培养液,即为检测样本;和(d)对所述检测样本进行基因检测,从而鉴定所述囊胚的健康状况。本发明提供的方法可去除IVF和ICSI技术过程中多余精子和母源颗粒细胞污染干扰的风险,并可精确的鉴定胚胎的健康状况。

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