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1. (CN108603222) Method for detecting target nucleic acid and nucleic acid probe used therein

Office : China
Application Number: 201780008629.2 Application Date: 06.02.2017
Publication Number: 108603222 Publication Date: 28.09.2018
Publication Kind : A
Prior PCT appl.: Application Number:PCTJP2017004191 ; Publication Number: Click to see the data
IPC:
C12Q 1/6813
C12N 15/09
G01N 21/78
[IPC code unknown for C12Q 1/6813]
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
75
Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77
by observing the effect on a chemical indicator
78
producing a change of colour
CPC:
C12Q 1/6876
C12N 15/09
C12Q 1/68
C12Q 1/6846
G01N 21/6428
G01N 21/78
G01N 2021/6432
G01N 2021/6439
Applicants: EIKEN KAGAKU KABUSHIKI KAISHA
荣研化学株式会社
Inventors: SAEKI RYOHEI
佐伯辽平
Agents: 永新专利商标代理有限公司 72002
Priority Data: 2016-022659 09.02.2016 JP
Title: (EN) Method for detecting target nucleic acid and nucleic acid probe used therein
(ZH) 对目标核酸进行检测的方法及该方法中使用的核酸探针
Abstract: front page image
(EN) The present invention is, in one embodiment, a nucleic acid probe that detects a target nucleic acid. At least one terminal of the probe-binding region of the target nucleic acid is a guanine base, and at least one cytosine base is present in the probe-binding region within 1 to 7 bases from the guanine base. The nucleic acid probe hybridizes with the probe-binding region of the target nucleic acid. The nucleic acid probe is provided with an oligonucleotide having at a terminal thereof a cytosine base opposite from the guanine base and is also provided with a fluorescent dye bonded to the cytosine base. The fluorescent dye is a fluorescent dye that is quenched by interaction with guanine base. The oligonucleotide is completely complementary with the nucleic acid of the probe-binding regionexcluding the cytosine base present within 1 to 7 bases from the terminal guanine base, and the base in the oligonucleotide opposite from the cytosine base that, of the cytosine bases, is nearest tothe terminal guanine base, is a base lacking a fluorescence quenching activity.
(ZH) 本发明的一个实施方式为对目标核酸进行检测的核酸探针。目标核酸的探针结合区域的至少一个末端为鸟嘌呤碱基、且在距离该鸟嘌呤碱基为1~7个碱基以内的所述探针结合区域存在1个以上的胞嘧啶碱基。核酸探针与目标核酸的探针结合区域杂交。核酸探针具备末端具有与该鸟嘌呤碱基相对的胞嘧啶碱基的寡核苷酸和结合于该胞嘧啶碱基的荧光色素。该荧光色素是在与鸟嘌呤碱基的相互作用下发生消光的荧光色素。所述寡核苷酸除了存在于距离所述末端鸟嘌呤碱基为1~7个碱基以内的胞嘧啶碱基之外、与所述探针结合区域的核酸完全互补,与该胞嘧啶碱基中最靠近所述末端鸟嘌呤碱基的胞嘧啶碱基相对的所述寡核苷酸上的碱基是不具有荧光的消光作用的碱基。
Also published as:
CA3012815EP3415636US20190055600WO/2017/138484