(EN) The invention discloses application of a crRNA mediated CRISPR/Cas13a gene editing system in tumor cells. According to the invention, in a U87 glioma cell system, a Cas13a protein can be mediated ontocomplementary target RNA by single-stranded crRNA and is cut. Meanwhile, the Cas13a protein can also trigger a joint shearing effect in eucaryotic cells, i.e., after starting to cut a first piece oftarget RNA, the Cas13a protein carries out aimless random cutting on other encountered RNA so as to take effects of inhibiting and killing tumors, such as effects of reducing a tumor cell index, inhibiting a mouse tumor formation rate and a tumor size. The invention provides a novel method for inhibiting or killing the tumor cells so as to lay a foundation for application of a random shearing effect of the CRISPR-Cas13 system in eukaryotic cells.
(ZH) 本发明公开了一种crRNA介导的CRISPR/Cas13a基因编辑系统在肿瘤细胞中的应用。本发明的Cas13a蛋白在U87胶质瘤细胞系中,可以由单链的crRNA介导到互补的目的RNA上,并进行切割。同时,Cas13a蛋白还能够在真核生物细胞中触发连带剪切的效应,即在开始切割第一条目的RNA之后,Cas13a蛋白对其他遇到的RNA,进行无目的的随机切割,从而起到降低肿瘤细胞指数,抑制小鼠成瘤率和肿瘤大小等抑制和杀伤肿瘤的作用。本发明提供了一种抑制或杀伤肿瘤细胞的新方法,为CRISPR‑Cas13系统随机剪切效应在真核细胞中的应用奠定了基础。