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1. CN108546718 - Application of crRNA mediated CRISPR/Cas13a gene editing system in tumor cells

Office
China
Application Number 201810465791.3
Application Date 16.05.2018
Publication Number 108546718
Publication Date 18.09.2018
Grant Number 108546718
Grant Date 09.07.2021
Publication Kind B
IPC
C12N 15/90
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90Stable introduction of foreign DNA into chromosome
C12N 15/113
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
CPC
C12N 15/113
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
C12N 15/907
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90Stable introduction of foreign DNA into chromosome
902using homologous recombination
907in mammalian cells
C12N 2310/10
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2310Structure or type of the nucleic acid
10Type of nucleic acid
C12N 2310/20
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2310Structure or type of the nucleic acid
10Type of nucleic acid
20involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Applicants KANG CHUNSHENG
康春生
Inventors KANG CHUNSHENG
康春生
WANG QIXUE
王琦雪
ZHOU JUNHU
周俊虎
Agents 天津市尚仪知识产权代理事务所(普通合伙) 12217
Title
(EN) Application of crRNA mediated CRISPR/Cas13a gene editing system in tumor cells
(ZH) crRNA介导的CRISPR/Cas13a基因编辑系统在肿瘤细胞中的应用
Abstract
(EN) The invention discloses application of a crRNA mediated CRISPR/Cas13a gene editing system in tumor cells. According to the invention, in a U87 glioma cell system, a Cas13a protein can be mediated ontocomplementary target RNA by single-stranded crRNA and is cut. Meanwhile, the Cas13a protein can also trigger a joint shearing effect in eucaryotic cells, i.e., after starting to cut a first piece oftarget RNA, the Cas13a protein carries out aimless random cutting on other encountered RNA so as to take effects of inhibiting and killing tumors, such as effects of reducing a tumor cell index, inhibiting a mouse tumor formation rate and a tumor size. The invention provides a novel method for inhibiting or killing the tumor cells so as to lay a foundation for application of a random shearing effect of the CRISPR-Cas13 system in eukaryotic cells.
(ZH) 本发明公开了一种crRNA介导的CRISPR/Cas13a基因编辑系统在肿瘤细胞中的应用。本发明的Cas13a蛋白在U87胶质瘤细胞系中,可以由单链的crRNA介导到互补的目的RNA上,并进行切割。同时,Cas13a蛋白还能够在真核生物细胞中触发连带剪切的效应,即在开始切割第一条目的RNA之后,Cas13a蛋白对其他遇到的RNA,进行无目的的随机切割,从而起到降低肿瘤细胞指数,抑制小鼠成瘤率和肿瘤大小等抑制和杀伤肿瘤的作用。本发明提供了一种抑制或杀伤肿瘤细胞的新方法,为CRISPR‑Cas13系统随机剪切效应在真核细胞中的应用奠定了基础。
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