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1. CN107849522 - Methods and microorganisms for the production of 1,3-butanediol

Office China
Application Number 201680042192.X
Application Date 21.07.2016
Publication Number 107849522
Publication Date 27.03.2018
Publication Kind A
IPC
C12N 1/21
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NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
C12N 15/52
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52Genes encoding for enzymes or proenzymes
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53Oxidoreductases (1)
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60Lyases (4)
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02Oxidoreductases (1.), e.g. luciferase
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0006acting on CH-OH groups as donors (1.1)
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7Preparation of oxygen-containing organic compounds
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04acyclic
18polyhydric
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C12Y 101/01001
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YENZYMES
101Oxidoreductases acting on the CH-OH group of donors (1.1)
01with NAD+ or NADP+ as acceptor (1.1.1)
01001Alcohol dehydrogenase (1.1.1.1)
Applicants GOVERNING COUNCIL OF THE UNIV OF TORONTO
多伦多大学管理委员会
Inventors MAHADEVAN RADHAKRISHNAN
R·马哈德万
YAKUNIN ALEXANDER
A·雅库宁
GAWAND PRATISH
P·加万德
NEMR KAYLA
K·内马尔
Agents 北京市铸成律师事务所 11313
北京市铸成律师事务所 11313
Priority Data 62/195,011 21.07.2015 US
Title
(EN) Methods and microorganisms for the production of 1,3-butanediol
(ZH) 用于产生1,3‑丁二醇的方法和微生物
Abstract
(EN)
A non-naturally occurring microorganism having a 1,3-BDO pathway is provided. The microorganism expresses at least one of the following 1,3-BDO pathway enzymes: an aldolase that catalyzes condensationof two acetaldehydes to produce 3-hydroxybutanal; and an aldo- ketoreductase, oxidoreductase, aldehyde reductase or alcohol dehydrogenase that reduces 3- hydroxybutanal to 1,3-BDO. The organism may further express one or more enzymes for producing acetaldehyde. A biosynthetic process involves condensing two acetaldehyde molecules to 3-hydroxybutanal using an enzyme from class aldolases; and selectively reducing 3-hydroxybutanal to 1,3-BDO using an enzyme belonging to the class aldo-ketoreductase, oxidoreductase, aldehyde reductase or alcohol dehydrogenase. The process can further include producing acetaldehyde by a biosynthetic method.

(ZH)
提供具有1,3‑BDO途径的非天然存在的微生物。所述微生物表达以下1,3‑BDO途径酶中的至少一种:催化两个乙醛缩合以产生3‑羟基丁醛的醛缩酶;和将3‑羟基丁醛还原为1,3‑BDO的醛酮还原酶、氧化还原酶、醛还原酶或醇脱氢酶。所述生物还可表达一种或多种用于产生乙醛的酶。生物合成工艺涉及使用来自醛缩酶类别的酶将两个乙醛分子缩合为3‑羟基丁醛;和使用属于醛酮还原酶类别、氧化还原酶类别、醛还原酶类别或醇脱氢酶类别的酶将3‑羟基丁醛选择性还原为1,3‑BDO。本工艺还可包括通过生物合成方法产生乙醛。