(EN) The invention provides a culture medium for the induced differentiation of functional cerebral cortical cells. The culture medium is prepared from a nerve cell culture medium and a nutrient additive,wherein the nutrient additive is selected from one or more of SU5402, BIBF1120, IBMX and glucose. The invention provides an induced differentiation method of the functional cerebral cortical cells. Specific factors such as an inhibitor of FGF and VEGF signal channels and an activator of cAMP are added in a specific time of the induced differentiation of various nerve cells from the nerve stem cells or nerve precursor cells to accelerate the differentiation and maturation of the nerve cells, the stable and healthy nerve cells having a main function can be obtained in about 7 to 14 days of the induced differentiation from the human nerve precursor cells, and the nerve cells comprise excitable nerve cells and inhibitory nerve cells, so that the in-vivo cultured human nerve cells can be actually applied to the field of the drug screening and the like, the production cost can be reduced, and the production period can be shortened.
(ZH) 本发明提供了一种功能大脑皮层细胞诱导分化用培养基，包括神经细胞培养基和营养添加剂，所述营养添加剂选自SU5402、BIBF1120、IBMX和葡萄糖中的一种或多种。本发明提供了一种功能大脑皮层细胞的诱导分化方法。本发明在由神经干细胞或神经前体细胞向多种神经细胞诱导分化的特定时间添加特定的因子，例如FGF、VEGF信号通路的抑制剂，cAMP的激活剂等，加速神经细胞的分化和成熟，可以在从人神经前体细胞开始诱导分化7～14天左右得到具有主要功能且稳定健康的神经细胞，其中包含兴奋性及抑制性神经元，从而使得体外培养的人神经细胞可以真正应用于药物筛选等领域，降低制造成本，缩短生产周期。
