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1. (CN108368549) Method for determining cell clonality

Office : China
Application Number: 201680071052.5 Application Date: 01.12.2016
Publication Number: 108368549 Publication Date: 03.08.2018
Publication Kind : A
Prior PCT appl.: Application Number:PCTEP2016079427 ; Publication Number: Click to see the data
IPC:
C12Q 1/6881
[IPC code unknown for C12Q 1/6881]
CPC:
C12Q 1/6874
C12Q 1/6881
Applicants: ARES TRADING S.A.
阿瑞斯贸易股份公司
Inventors: LA NEVE FABIO
F·拉内韦
FEGER GEORG
G·菲格
TOSO EMILIANO
E·托索
Agents: 隆天知识产权代理有限公司 72003
Priority Data: 15197894.7 03.12.2015 EP
Title: (EN) Method for determining cell clonality
(ZH) 确定细胞克隆形成能力的方法
Abstract: front page image
(EN) The invention relates to a method for determining the clonality of a master cell bank (MCB) in which a transgene has been inserted. The method involves a combination of sequencing methodology and bioinformatic analysis, performed on multiple subclones originating from a common MCB, to establish a reliable set of reference transgene insertion regions in one reference subclone, and corresponding sets of comparative transgene insertion regions in one or more other subclones originating from the same MCB. Based on the degree of congruity between reference and comparative transgene insertion regions, the MCB is determined to be either monoclonal or polyclonal.
(ZH) 本发明涉及一种确定其中已经插入转基因的主细胞库(MCB)的克隆形成能力的方法。该方法包括测序方法和生物信息学分析的组合,其在源自共同MCB的多个亚克隆上执行,从而在一个参考亚克隆中建立可靠的一组参考转基因插入区域并在源自相同MCB的一个或多个其他亚克隆中建立相应的多组对比性转基因插入区域。基于参考和对比性转基因插入区域之间的一致程度,确定MCB为单克隆或多克隆。
Also published as:
AU2016363746CA3005386KR1020180088867EP3384045US20180327830IL259750
WO/2017/093400