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1. CN108254562 - Time-resolved fluorescence immunochromatographic test strip and kit for detection of MYO, and preparation methods thereof

Office
China
Application Number 201611237909.4
Application Date 28.12.2016
Publication Number 108254562
Publication Date 06.07.2018
Grant Number 108254562
Grant Date 08.11.2019
Publication Kind B
IPC
G01N 33/68
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/577
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
577involving monoclonal antibodies
G01N 33/558
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
558using diffusion or migration of antigen or antibody
G01N 21/64
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
CPC
G01N 21/6408
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
6408with measurement of decay time, time resolved fluorescence
Applicants RAYBIOTECH, INC. GUANGZHOU
广州瑞博奥生物科技有限公司
Inventors XU BUZHUO
徐部灼
SONG XUDONG
宋旭东
HUANG RUOPAN
黄若磐
Agents 广州华进联合专利商标代理有限公司 44224
广州华进联合专利商标代理有限公司 44224
Title
(EN) Time-resolved fluorescence immunochromatographic test strip and kit for detection of MYO, and preparation methods thereof
(ZH) 检测MYO的时间分辨荧光免疫层析试纸条、试剂盒及其制备方法
Abstract
(EN)
The invention discloses a time-resolved fluorescence immunochromatographic test strip and kit for detection of MYO, and preparation methods thereof. The test strip comprises a bottom liner and furthercomprises a sample pad, a conjugate pad, a coating membrane and absorbent paper successively arranged on the bottom liner, wherein the conjugate pad is coated with a MYO monoclonal detection antibodylabeled by fluorescence microspheres; the coating membrane comprises a test zone and a control zone arranged in parallel and spaced apart from each other; the test zone is coated with a MYO monoclonal capture antibody capable of recognizing a single epitope, and the control zone is coated with a goat anti-mouse IgG antibody; and the coating membrane a nitrocellulose membrane bonded with a polymer, and the polymer is a material having light transmittance of 10% or below under irradiation of light with wavelengths of less than 450 nm and light transmittance of 95% or more under irradiation of light with wavelengths of 500 nm or more. The test strip of the invention can realize quick and quantitative detection, and is accurate and reliable in test results and high in sensitivity; the preparation method is simple in process and suitable for large-scale production; and the test strip has positive significance to quantitative detection of MYO.

(ZH)
本发明公开了一种检测MYO的时间分辨免疫层析试纸条、试剂盒及其制备方法,所述试纸条包括底衬、以及依次设在所述底衬上的样品垫、结合垫、包被膜和吸水纸,所述结合垫上包被有荧光微球标记的MYO单克隆检测抗体,所述包被膜包括平行设置、且相互间隔的检测区和对照区,所述检测区包被有识别单一抗原表位的MYO单克隆捕获抗体,所述对照区包被有羊抗鼠IgG抗体;所述包被膜为结合有聚合物的硝酸纤维膜,所述聚合物为在小于450nm波长具有10%以下透光率,在500nm波长以上具有95%以上的透光率的材料。本发明的试纸条,可以快速定量检测,试验结果准确可靠,灵敏度高,制备方法简单,适合大规模生产,对MYO定量检测有积极意义。

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