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1. (CA2613471) CELL LINES FOR EXPRESSING ENZYME USEFUL IN THE PREPARATION OF AMIDATED PRODUCTS

Office : Canada
Application Number: 2613471 Application Date: 23.06.2006
Publication Number: 2613471 Publication Date: 04.01.2007
Grant Number: Grant Date: 06.09.2016
Publication Kind : C
Prior PCT appl.: Application Number:PCTUS2006024687 ; Publication Number: Click to see the data
IPC:
C12P 21/04
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
04
Cyclic or bridged peptides or polypeptides, e.g. bacitracin
CPC:
C12P 21/00
C12N 9/0071
Applicants: UNIGENE LABORATORIES, INC.
Inventors:
Priority Data: 60/693,612 24.06.2005 US
Title: (EN) CELL LINES FOR EXPRESSING ENZYME USEFUL IN THE PREPARATION OF AMIDATED PRODUCTS
(FR) LIGNEES CELLULAIRES PERMETTANT D'EXPRIMER UNE ENZYME UTILE POUR LA PREPARATION DE PRODUITS AMIDES
Abstract: front page image
(EN)


Cell lines are provided for expressing peptidylglycine alpha-amidating
monooxygenase (PAM), or one of its two catalytic domains. High levels of
enzyme expression are achieved while utilizing a non-animal source, low
protein tissue culture medium. A robust two-step downstream purification
results in high enzyme purity. Resulting PAM, or its PHM catalytic domain, is
used to catalyze the enzymatic conversion of X-Gly to X-alpha-hydroxy-Gly or X-
NH2 (X being a peptide or any chemical compound having a carbonyl group to
which a glycine group can be covalently attached). Methods of preparing
preferred cell lines are also set forth.


Also published as:
EP1907561JP2008543344JP2011139714JP2012016354CN101287840ES2437068
AU2006261909DK1907561WO/2007/002532