CA2493240 METHODS FOR DETECTING OR ASSAYING VIRUS

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Application Number 2493240

Application Date 04.08.1998

Publication Number 2493240

Publication Date 11.02.1999

Publication Kind A1

IPC

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Applicants ADVANCED LIFE SCIENCE INSTITUTE, INC.

Inventors AOYAGI, KATSUMI
OHUE, CHIHARU
IIDA, KUMIKO
KIMURA, TATSUJI
YAGI, SHINTARO

Priority Data 1998218136 31.07.1998 JP
1997209515 04.08.1997 JP
9/209522 04.08.1997 JP

Title

(EN) METHODS FOR DETECTING OR ASSAYING VIRUS

(FR) METHODES PERMETTANT DE DETECTER OU D'ANALYSER UN VIRUS

Abstract

(EN) A method for treating a hepatitis C or hepatitis B virus-containing sample, characterized by treatment of the virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant, and a virus assay method using said treating method. Also provided is a method for treating a hepatitis C virus-containing sample, characterized by treatment of the virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent, and a virus assay method using said treating method. Further provided is a hepatitis C virus assay method comprising treating a hepatitis C virus antigen-containing sample with a surfactant having an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them, and measuring binding of the antigen with a hepatitis C virus antibody. Finally, there is also provided a monoclonal antibody and a hybridoma producing the same for carrying out the above methods.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5	Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10	Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
12	Fused cells, e.g. hybridomas

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
K	PEPTIDES
16	Immunoglobulins, e.g. monoclonal or polyclonal antibodies
08	against material from viruses
10	from RNA viruses

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7	Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
04	Inactivation or attenuation; Producing viral sub-units
06	by chemical treatment

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
569	for microorganisms, e.g. protozoa, bacteria, viruses

G	PHYSICS
01	MEASURING; TESTING
N	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33	Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48	Biological material, e.g. blood, urine; Haemocytometers
50	Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53	Immunoassay; Biospecific binding assay; Materials therefor
576	for hepatitis

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1. CA2493240 - METHODS FOR DETECTING OR ASSAYING VIRUS