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1. (CA2073043) IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS

Office : Canada
Application Number: 2073043 Application Date: 07.11.1990
Publication Number: 2073043 Publication Date: 08.05.1991
Grant Number: Grant Date: 21.12.1999
Publication Kind : C
Prior PCT appl.: Application Number:US1990006449 ; Publication Number: Click to see the data
IPC:
C12N 9/02
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02
Oxidoreductases (1.), e.g. luciferase
CPC:
C12N 9/0089
Applicants:
Inventors: BARTFELD, DANIEL
LIFSHITZ, RUTH
HADARY, DANY
Priority Data: 432,871 07.11.1989 US
Title: (EN) IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS
(FR) METHODE DE PURIFICATION DE LA CUIVRE/ZINC (CU-ZN) SUPEROXYDE DISMUTASE OBTENUE A PARTIR DE RECOMBINANTS DE BACTERIES OU DE CELLULES D'EUCARYOTES
Abstract: front page image
(EN) The subject invention provides a method for recovering a solution containing purified, enzymatically active Cu-Zn superoxide dismutase or a polypeptide analog thereof having substantially the same amino acid sequence as, and the biological activity of, naturally-occurring Cu-Zn superoxide dismutase from a composition which comprises cells containing Cu-Zn superoxide dismutase or a polypeptide analog thereof. The invention also provides a method of increasing the yield of recovered solutions having an increased concentration of b isoform of an enzymatically-active polypeptide analog of Cu-Zn superoxide dismutase from a composition which comprises cells containing a, b and c isoforms of the polypeptide analog.


Also published as:
IL96136EP0499621AU1991068718WO/1991/006634