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1. CA3013946 - METHOD AND SYSTEM FOR IMPROVING LATERAL RESOLUTION IN OPTICAL SCANNING MICROSCOPY

Office Canada
Application Number 3013946
Application Date
Publication Number 3013946
Publication Date 24.08.2017
Publication Kind A1
IPC
G PHYSICS
02
OPTICS
B
OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21
Microscopes
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
483
Physical analysis of biological material
G PHYSICS
02
OPTICS
B
OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21
Microscopes
06
Means for illuminating specimen
G02B 21/00
G01N 33/483
G02B 21/06
CPC
G02B 5/001
G02B 21/0032
G02B 21/0056
G02B 27/0927
G02B 27/095
G02B 27/0988
Applicants UNIVERSITE LAVAL
Priority Data 62/295,819 16.02.2016 US
Title
(EN) METHOD AND SYSTEM FOR IMPROVING LATERAL RESOLUTION IN OPTICAL SCANNING MICROSCOPY
(FR) PROCEDE ET SYSTEME D'AMELIORATION DE RESOLUTION LATERALE DE MICROSCOPIE A BALAYAGE OPTIQUE
Abstract
(EN)
A method and system for improving lateral resolution in optical microscopy are provided. The method includes generating a source optical beam and passing the source optical beam successively through an axicon, a Fourier-transform lens and an objective to convert the source optical beam into an excitation Bessel-type beam having a central lobe and at least one side lobe. The method also includes focusing the excitation beam onto a focal plane of the objective within or on a sample to generate a sample light signal, and spatially filtering the sample light signal. The spatial filtering includes rejecting light originating from outside of the focal plane and light generated by the at least one side lobe of the excitation beam. The spatial filtering also includes permitting passage, as a filtered light signal, of light generated by the central lobe of the excitation beam. The method further includes detecting the filtered light signal.

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