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1. CA3012815 - METHOD FOR DETECTING TARGET NUCLEIC ACID AND NUCLEIC ACID PROBE USED THEREIN

Office Canada
Application Number 3012815
Application Date
Publication Number 3012815
Publication Date 17.08.2017
Publication Kind A1
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
75
Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
77
by observing the effect on a chemical indicator
78
producing a change of colour
C12Q 1/68
C12N 15/09
G01N 21/78
CPC
C12Q 1/6846
C12N 15/09
C12Q 1/68
G01N 21/78
C12Q 1/6876
G01N 21/6428
Applicants EIKEN KAGAKU KABUSHIKI KAISHA
Priority Data 2016-022659 09.02.2016 JP
Title
(EN) METHOD FOR DETECTING TARGET NUCLEIC ACID AND NUCLEIC ACID PROBE USED THEREIN
(FR) PROCEDE DE DETECTION D'ACIDE NUCLEIQUE CIBLE ET SONDE D'ACIDE NUCLEIQUE UTILISEE DANS CE PROCEDE
Abstract
(EN)
The present invention is, in one embodiment, a nucleic acid probe that detects a target nucleic acid. At least one terminal of the probe-binding region of the target nucleic acid is a guanine base, and at least one cytosine base is present in the probe-binding region within 1 to 7 bases from the guanine base. The nucleic acid probe hybridizes with the probe-binding region of the target nucleic acid. The nucleic acid probe is provided with an oligonucleotide having at a terminal thereof a cytosine base opposite from the guanine base and is also provided with a fluorescent dye bonded to the cytosine base. The fluorescent dye is a fluorescent dye that is quenched by interaction with guanine base. The oligonucleotide is completely complementary with the nucleic acid of the probe-binding region excluding the cytosine base present within 1 to 7 bases from the terminal guanine base, and the base in the oligonucleotide opposite from the cytosine base that, of the cytosine bases, is nearest to the terminal guanine base, is a base lacking a fluorescence quenching activity.