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1. CA2996711 - METHOD OF FERMENTATIVE ALPHA-IONONE PRODUCTION

Office
Canada
Application Number 2996711
Application Date
Publication Number 2996711
Publication Date 09.03.2017
Grant Number
Grant Date 28.09.2021
Publication Kind C
IPC
C12P 23/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
23Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
C12N 15/63
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12P 5/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
5Preparation of hydrocarbons
CPC
C12N 9/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
C12N 9/90
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
90Isomerases (5.)
C12P 5/007
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
5Preparation of hydrocarbons ; or halogenated hydrocarbons
007containing one or more isoprene units, i.e. terpenes
C12P 23/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
23Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
C12Y 103/9903
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
103Oxidoreductases acting on the CH-CH group of donors (1.3)
99with other acceptors (1.3.99)
9903Phytoene desaturase (3,4-didehydrolycopene-forming) (1.3.99.30)
C12Y 104/99
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
104Oxidoreductases acting on the CH-NH2 group of donors (1.4)
99with other acceptors (1.4.99)
Applicants PHYTOWELT GREENTECHNOLOGIES GMBH
Title
(EN) METHOD OF FERMENTATIVE ALPHA-IONONE PRODUCTION
(FR) PROCEDE DE PRODUCTION PAR FERMENTATION D'ALPHA-IONS
Abstract
(EN) The present invention relates to a method of preparing enantiomerically pure alpha-ionone. The invention further relates to a nucleic acid comprising a sequence that encodes a lycopene epsilon-cyclase (EC), a lycopene epsilon-cyclase (EC), plasmids that encode components of alpha-ionone biosynthesis and a microorganism containing heterologous nucleotide sequences that encode the enzymes geranylgeranyl diphosphate synthase, isopentenyl diphosphate isomerase (IPI), phytoene desaturase/dehydrogenase (crtl), phytoene synthase (crt B) and lycopene epsilon-cyclase (EC) or geranylgeranyl diphosphate synthase, isopentenyl diphosphate isomerase (IPI), phytoene desaturase/dehydrogenase (crtl), phytoene synthase (crt B), lycopene epsilon-cyclase (EC) and carotenoid cleavage dioxygenase (CCD1). The invention further relates to a method of producing high-purity epsilon-carotene.