(EN) The invention provides a method for regulated manipulation of a biological sample. The method includes selecting the biological sample, initiating a first manipulation on the selected biological sample and performing a second manipulation, subsequent to the first manipulation. The invention also provides an automated system for regulated manipulation of a biological sample. The system includes a stage for positioning the sample, a sensing means coupled to the stage, a first manipulation device positioned in the plane of the stage for a first manipulation of the biological sample, a second manipulation device positioned in-plane or out of plane of the stage for a second manipulation of the biological sample and an analyzer coupled to each of the first manipulation device and the second manipulation device. The coupling enables the analyzer to independently regulate the first manipulation device, the second manipulation device and a combination thereof.