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1. AU2008222611 - Vectors and methods for enzyme production

Office
Australia
Application Number 2008222611
Application Date 06.03.2008
Publication Number 2008222611
Publication Date 01.10.2009
Publication Kind A1
IPC
C12N 15/52
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
C12N 9/00
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
C12N 15/40
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
31Genes encoding microbial proteins, e.g. enterotoxins
33Genes encoding viral proteins
40Proteins from RNA viruses, e.g. flaviviruses
C12N 15/63
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
CPC
C12N 9/127
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9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1241Nucleotidyltransferases (2.7.7)
127RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
C12N 15/67
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15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67General methods for enhancing the expression
C12N 2795/18122
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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2795Bacteriophages
00011Bacteriophages
18011ssRNA Bacteriophages positive-sense
18111Leviviridae
18122New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
C12N 2800/101
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2800Nucleic acids vectors
10Plasmid DNA
101for bacteria
C12N 2800/40
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2800Nucleic acids vectors
40Systems of functionally co-operating vectors
C12N 2830/002
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2830Vector systems having a special element relevant for transcription
001controllable enhancer/promoter combination
002inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
Applicants Macquarie University
Inventors Bergquist, Peter Leonard
Sunna, Anwar
Agents Davies Collison Cave Pty Ltd
Priority Data 2007901150 06.03.2007 AU
Title
(EN) Vectors and methods for enzyme production
Abstract
(EN) The invention provides a method for producing a replicase comprising EF-Ts, EF-Tu and β polypeptide subunits, the method comprising expressing in a suitable host cell the EF-Ts and EF-Tu subunits, followed by expressing the β subunit, wherein the EF-Ts and EF-Tu subunits are operably linked to a first promoter and the β subunit is operably linked to a second promoter, and wherein the first and second promoters are differentially induced.