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1. (AU1990066412) Method for reproducing conifers by somatic embryogenesis

Office : Australia
Application Number: 66412/90 Application Date: 22.10.1990
Publication Number: 1990066412 Publication Date: 01.12.1994
Publication Kind : A
Prior PCT appl.: Application Number:US1990006057 ; Publication Number: Click to see the data
IPC:
C12N 5/00
C12N 5/04
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
04
Plant cells or tissues
CPC:
C12N 5/04
C12N 5/0025
Applicants: Weyerhaeuser Company
Inventors: Gupta, Pramod K.
Pullman, Gerald S.
Agents: PHILLIPS ORMONDE FITZPATRICK
Priority Data: 426331 23.10.1989 US
499151 26.03.1990 US
Title: (EN) Method for reproducing conifers by somatic embryogenesis
Abstract:
(EN) The invention is a method for reproducing coniferous trees by somatic embryogenesis using plant tissue culture. It comprises a multistage process in which a fertilized immature-embryo is excised and cultured to induce early state proembryos, which may be further cultured in liquid media without auxins or cytokinins but with the addition of abscisic acid, to develop late stage proembryos under significantly higher osmotic conditions of about 450 mM/kg. The medium may have added exogenous abscisic acid and must contain an absorbent material. After several weeks in culture the vigor and morphology of the embryos developed by the process are improved and the tendency of premature germination is reduced. The somatic embryos may be germinated before or after storage and transplanted to soil for further growth.
Also published as:
CA2069964WO/1991/005854