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1. CN103555783 - Method for preparing phosphatidyserine

Office
China
Application Number 201310508296.3
Application Date 24.10.2013
Publication Number 103555783
Publication Date 05.02.2014
Grant Number 103555783
Grant Date 12.08.2015
Publication Kind B
IPC
C12P 13/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
04Alpha- or beta-amino acids
06Alanine; Leucine; Isoleucine; Serine; Homoserine
Applicants 陕西源邦生物技术有限公司
Inventors 李艳
赵江
张永利
寇玉锋
Agents 西安智邦专利商标代理有限公司 61211
Title
(EN) Method for preparing phosphatidyserine
(ZH) 一种制备磷脂酰丝氨酸的方法
Abstract
(EN)
The invention relates to a method for preparing phosphatidyserine. The method for preparing phosphatidyserine comprises the following steps of mixing phosphatidylcholine or natural phospholipid containing phosphatidylcholine with tween-80, L-serine, calcium chloride, buffer solution and phospholipase D, and stirring and reacting the above materials at 35-45 DEG C for 8-10h; separating out the crude products from reaction and floating, carrying out centrifugal separation on the aqueous phase and crude products, washing the crude products subjected to separation and carrying out centrifugal filtration; adding the crude products subjected to centrifugal filtration into absolute ethyl alcohol for immersing, stirring, and carrying out centrifugal filtration; and carrying out vacuum drying on the products subjected to centrifugal filtration to obtain the phosphatidyserine. The invention aims to provide a new method for preparing phosphatidyserine to solve the problems that in the prior art, the byproduct of phosphatidic acid is large in amount, the conversion rate is unsatisfactory and the product cost is high.

(ZH)

一种制备磷脂酰丝氨酸的方法,包括以下步骤:将磷脂酰胆碱或含有磷脂酰胆碱的天然磷脂与吐温-80、L-丝氨酸、氯化钙、缓冲溶液、磷脂酶D混合,在35-45℃搅拌反应8-10h;反应结束后反应粗品析出并上浮,离心分离水相和反应粗品,将分离得到的反应粗品用水洗涤,并离心过滤;离心过滤后的反应粗品加入无水乙醇浸泡,搅拌,再离心过滤;对离心过滤后产物真空干燥,即得磷脂酰丝氨酸产品。本发明的目的是提供一种新的制备磷脂酰丝氨酸的方法,以解决现有技术存在的副产物磷脂酸较多,转化率不理想,生产成本高的问题。