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1. WO2010088219 - REUSABLE BIOSENSOR PLATFORM

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[ EN ]

CLAIMS What is claimed is:

1. A microfluidic device comprising: a substrate layer; an electrode layer disposed above the substrate layer; a spacer layer disposed above the electrode layers to form at least two channels; another electrode layer disposed above the spacer layer; and another substrate layer disposed above the other electrode layer, wherein the other substrate layer comprises at least two inlets, each connected to one of the at least two channels, two outlets corresponding to the two inlets, and two electrical contacts filled with a conductive material to form a conductive feedthrough to the other electrode layer.

2. The microfluidic device of claim 1 , wherein the different layers are bonded together by using oxygen plasma or adhesives.

3. The microfluidic device of claim 1 , wherein the substrate layer and the other substrate layer comprise at least one of glass, silicon and plastic substrate layers.

4. The microfluidic device of claim 1 , wherein the electrode layer and the other electrode layer comprise one or more conducting films.

5. The microfluidic device of claim 1 , wherein the electrode layer and the other electrode layer comprise at least one of chromium and gold.

6. The microfluidic device of claim 1 , wherein the electrical contacts are filled with a conducting material.

7. The microfluidic device of claim 6, wherein the conducting material comprises silver paste.

8. The microfluidic device of claim 1 , wherein one of the two inlets is connected to a reference channel to be used in reference measurements and the other inlet is connected to a sample inlet to receive a sample protein and to be used in sample measurements.

9. The microfluidic device of claim 8, wherein a surface plasmon resonance (SPR) analytical system is connected to the microfluidic device to record SPR angle shift in real time comprising: the reference channel to receive a reference solution comprising a self-assembled-monolayer (SAM) to modify a surface of the electrode layer in the reference channel; the sample channel to receive a sample solution comprising the SAM to modify a surface of the electrode layer in the sample channel, and a target molecule that immobilizes on the SAM; and the SPR analytical system to apply a laser beam and measure a differential measurement of an angle shift of the applied laser beam incident on the modified surface of the electrode layer in the reference channel without the target molecule immobilized on the SAM and an angle shift of the applied laser beam incident on the modified surface of the electrode layer in the sample channel with the target molecule immobilized on the SAM.

10. The microfluidic device of claim 9, wherein the microfluidic device is configured to be reusable by desorbing the SAM and the target molecule from the surface of the electrode layer in the sample channel responsive to a reductive voltage applied to the electrode layer in the sample channel.

1 1. The microfluidic device of claim 10, wherein the reductive voltage applied is based on at least one of a length of an alkyl chain, a type of terminal group, or binding of proteins.

12. The microfluidic device of claim 1 , wherein the electrode layer comprises a cathode to receive a positive potential; and the other electrode layer comprises an anode to receive a negative potential.

13. The microfluidic device of claim 1 , wherein the electrode layer and the other electrode layer are connected to a potentiostat device to conduct ex-situ electrochemical measurement using linear sweep voltammetry.

14. A method comprising: forming a self-assembly monolayer on a metal surface of a microfluidic device that comprises a reference channel, a sample channel, a cathode and an anode, wherein the forming comprises: flowing a reference solution comprising a self-assembled-monolayer

(SAM) through the reference channel and the sample channel to modify a metal surface of the cathode in the reference channel and the sample channel; flowing a target molecule through the sample channel to be immobilized on the SAM; detecting a presence of the SAM with the immobilized target molecule formed on the metal surface of the cathode; and applying a reductive potential to desorb the SAM along with the immobilized target molecule from the metal surface of the cathode in the sample channel.

15. The method of claim 14, comprising: after desorbing the target molecule bound SAM, reusing the microfluidic device by flowing another solution comprising another SAM to reconfigure the surface of the cathode.

16. The method of claim 15, comprising: flowing another target molecule through the sample channel to bind with the other SAM.

17. The method of claim 15, comprising: recording contact angle measurement on the other target molecule bound SAM; and applying the reductive potential to remove the other target molecule-bound SAM.

18. The method of claim 14, wherein the target molecule comprises streptavidin that immobilizes on the surface of the cathode.

19. The method of claim 14, wherein the cathode comprises gold electrode.

20. The method of claim 14, wherein the reductive potential applied is based on at least one of a length of an alkyl chain, a type of terminal group, or binding of proteins.

21. The method of claim 14, wherein detecting the presence of the SAM with the immobilized target molecule comprises: recording an angle shift of an applied laser beam incident on the metal surface of the cathode in the reference channel without the immobilized target molecule and the sample channel with the immobilized molecule in real time; and obtaining a differential measurement of the real time angle shift in the reference and sample channels.