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1. WO2003040297 - NEW PROTEIN OF THE DEFENSIN FAMILY FROM OIL PALM

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[ EN ]

CLAIMS
1) An isolated polypeptide of the defensin family, hereinafter referred as EGADl, having at least 70% sequence identity with the polypeptide of SEQ ID NO: 2.
5 2) A fragment of an EGADl polypeptide of claim 1, comprising at least 5 contiguous amino-acids of said polypeptide.
3) An isolated polynucleotide selected among :
a) a polynucleotide encoding an EGADl polypeptide L0 of claim 1;
b) a fragment of a polynucleotide a) comprising at least 10 contiguous nucleotides ;
c) a polynucleotide complementary of a polynucleotide a) or b) above;
.5 d) a polynucleotide hybridising selectively under stringent conditions with a polynucleotide a) b) or c) above.
4) A polynucleotide of claim 3 selected among :
a) the cDNA of SEQ ID NO:l;
b) a polynucleotide comprising at least 10 '0 contiguous nucleotides of SEQ ID NO: 1;
c) a polynucleotide complementary of a polynucleotide a) or b) above;
d) a polynucleotide hybridising selectively under stringent conditions with a polynucleotide a) b) or c) above.

5 5) An antibody directed against a polypeptide of any of claims 1 or 2.
6) The use of a polypeptide of any of claims 1 or 2 or of a polynucleotide of any of claims 3 or 4, or of an antibody of claim 5, for detecting the mantled phenotype in an

0 oil palm tissue culture.
7) A method for detecting the mantled abnormality in an oil palm tissue culture, wherein said method comprises detecting the overexpression of the EGADl gene, encoding an EGADl polypeptide of claim 1, in said culture.
5 8) A method of claim 7, wherein the overexpression of the EGADl gene is detected in an oil palm tissue culture at the callus stage.

9) A method of any of claims 7 or 8, wherein the overexpression of the EGADl gene is detected by evaluating the amount of EGADl transcripts.