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255. (WO2012045756) PROCESS FOR PRODUCING THREE-DIMENSIONAL TISSUE

Pub. No.:    WO/2012/045756    International Application No.:    PCT/EP2011/067344
Publication Date: Fri Apr 13 01:59:59 CEST 2012 International Filing Date: Wed Oct 05 01:59:59 CEST 2011
IPC: C12N 5/00
G01N 21/64
Applicants: MEDIZINISCHE HOCHSCHULE HANNOVER
BIOMIMETICS TECHNOLOGIES INC.
SCHMÄLZLIN, Elmar
STOLZ, Marvin
WEYAND, Birgit
ISRAELOWITZ, Meir
VOGT, Peter
REIMERS, Kerstin
VON SCHROEDER, Herbert, P.
GILLE, Christoph
Inventors: SCHMÄLZLIN, Elmar
WEYAND, Birgit
ISRAELOWITZ, Meir
VOGT, Peter
REIMERS, Kerstin
VON SCHROEDER, Herbert, P.
GILLE, Christoph
Title: PROCESS FOR PRODUCING THREE-DIMENSIONAL TISSUE
Abstract:
The invention provides a process for producing a three-dimensional tissue by cultivating eucaryotic cells by introducing the cells into a matrix and cultivating the cells within the matrix in a cell culture medium within a cell culture vessel under controlled dissolved oxygen conditions of the cell culture medium. The matrix contains a support containing an optical oxygen sensor, which is an oxygen-sensitive dye, which upon irradiation with an excitation wavelength changes its emission characteristics in dependence on the dissolved oxygen concentration in the surrounding medium, including a dye phosphorescing upon irradiation of an excitation wavelength, which phosphorescence is quenched by dissolved oxygen.