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1. WO2006073504 - WOBBLE SEQUENCING

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[ EN ]

What is claimed is:

1. A method described above for DNA sequencing, useful for sequencing homopolymeric regions of DNA.

2. A method of sequencing a target nucleic acid comprising:
a. providing a sequencing primer, wherein the sequencing primer has at least one anchor sequence and a universal base;
b. hybridizing the sequencing primer to a target nucleic acid; and
c. extending the sequencing primer.

3. A method of sequencing a target nucleic acid comprising:
a. providing a sequencing primer, wherein the sequencing primer has at least one anchor sequence and a degenerate base;
b. hybridizing the sequencing primer to a target nucleic acid; and
c. extending the sequencing primer.

4. A method of sequencing a target nucleic acid comprising:
a. providing a sequencing primer, wherein the sequencing primer has at least one anchor sequence and a natural base;
b. hybridizing the sequencing primer to a target nucleic acid; and
c. extending the sequencing primer.

5. A method for sequencing a target nucleic acid comprising:
(a) hybridization of one of several anchor primers to a common sequence adjacent to an unknown sequence,
(b) ligation of fluorescently labeled, degenerate oligonucleotides to the anchor primer, such that identity of the fluorophore is informative of the identity of one or more positions within the degenerate oligonucleotide,
(c) imaging to determine primer ligation,
(d) stripping of the anchor primeπdegenerate oligonucleotide complexes, and

(e) repeating steps (a)-(d) one or more times.