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1. EP3160980 - HYBRID tRNA/pre-miRNA MOLECULES AND METHODS OF USE

Office
European Patent Office
Application Number 15800458
Application Date 21.05.2015
Publication Number 3160980
Publication Date 03.05.2017
Publication Kind B1
IPC
C12N 15/11
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C12N 15/113
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
CPC
A61P 35/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
35Antineoplastic agents
A61P 35/02
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
35Antineoplastic agents
02specific for leukemia
C12N 15/11
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C12N 15/111
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
111General methods applicable to biologically active non-coding nucleic acids
C12N 2310/141
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2310Structure or type of the nucleic acid
10Type of nucleic acid
14interfering N.A.
141MicroRNAs, miRNAs
C12N 2310/3519
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2310Structure or type of the nucleic acid
30Chemical structure
35Nature of the modification
351Conjugate
3519Fusion with another nucleic acid
Applicants UNIV CALIFORNIA
Inventors YU AIMING
WANG WEIPENG
CHEN QIUXIA
LI MEIMEI
Designated States
Priority Data 201462003806 28.05.2014 US
Title
(DE) HYBRIDE TRNA/PRE-MIRNA-MOLEKÜLE UND VERFAHREN ZUR VERWENDUNG
(EN) HYBRID tRNA/pre-miRNA MOLECULES AND METHODS OF USE
(FR) MOLECULES HYBRIDES DE tARN/pre-miARN ET PROCEDES D'UTILISATION
Abstract
(EN)
Provided are hybrid tRNA/pre-microRNA and tRNA/shRNA molecules and methods of making and using. Accordingly, in one aspect, polynucleotides comprising a tRNA operably linked to a pre-microRNA (pre-miRNA) or short hairpin RNA (shRNA) are provided. In varying embodiments, the tRNA is a methionyl tRNA. In varying embodiments, the pre-miRNA (or shRNA) is selected from premiRNA-1291, pre-miRNA-34a, pre-miRNA- 125b, pre-miRNA- 124, pre-miRNA-27b, and pre-miRNA-22.

(FR)
L'invention concerne des molécules hybrides d'ARNt/pré-microARN et d'ARNt/ARNsh ainsi que des procédés de fabrication et d'utilisation correspondants. En conséquence, dans un aspect, elle se rapporte à des polynucléotides comprenant un ARNt lié fonctionnellement à un pré-microARN (pré-miARN) ou à un petit ARN en épingle à cheveux (ARNsh). Dans divers modes de réalisation, l'ARNt est le méthionyl-ARNt. Dans divers modes de réalisation, le pré-miARN (ou ARNsh) est choisi parmi le pré-miARN-1291, le pré-miARN-34a, le pré-miARN-125b, le pré-miARN-124, le pré-miARN-27 b et le pré-miARN-22.