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1. WO2020136237 - WEISSELLA VIRIDESCENS STRAIN AND USES THEREOF FOR THE SYNTHESIS OF EXOPOLYSACCHARIDES

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CLAIMS

1 . Bacterial strain comprising the SEQ ID NO: 1 .

2. The bacterial strain according to claim 1 wherein the SEQ ID NO: 1 is comprised in an extrachromosomal plasmid, preferably the extrachromosomal plasmid comprises the SEQ ID NO: 3.

3. The bacterial strain according to any of the claims 1 and 2 wherein the SEQ ID NO: 1 encodes an enzyme capable of producing exopolysaccharides, wherein preferably, the enzyme comprises the SEQ ID NO: 2.

4. The bacterial strain according to any of the claims 1 to 3 characterized by belonging to the genus Weissella, preferably belonging to the species Weissella viridescens, more preferably is the strain W. viridescens CECT 9735.

5. Nucleotide sequence comprising the SEQ ID NO: 1.

6. Polypeptide sequence encoded by the nucleotide sequence according to claim 5, wherein preferably the polypeptide sequence comprising the SEQ ID NO: 2.

7. Vector comprising the nucleotide sequence according to claim 5, wherein the vector is preferably a plasmid.

8. Plasmid comprising the SEQ ID NO: 3.

9. Host cell comprising the nucleotide sequence according to claim 5, the polypeptide sequence according to claim 6, the vector according to claim 7, or the plasmid according to claim 8.

10. Use of the bacterial strain according to any of the claims 1 to 4 or the host cell according to claim 9, for exopolysaccharide production.

1 1 . Method for producing exopolysaccharides comprising the following steps: a) culture the bacterial strain according to any of the claims 1 to 4, or the host cell according to claim 9, in a culture medium; preferably at a pH in the range from 6.5 to 7.5, preferably 7.2; and at a temperature in the range from 28°C to 35°C, preferably 30°C; and

b) extraction of the exopolysaccharides synthesized by the cell in step a).

12. Exopolysaccharide obtainable by the method according to claim 1 1 .

13. Exopolysaccharide characterised by comprising glucose, mannose and arabinose, in a molar ratio in the range from 3.1 : 1 : 0.1 , preferably wherein the exopolysaccharide comprising at least the following percentages by weight: 30-45% C; 1 -10%H; 1 -15% N and 0.05-2% S; and an average molecular weight in the range from 100 to 150 kDa, an average molecular mass by weight in the range from 50 to 100 kDa and a polydispersity index in the range from 1 .5 and 2.

14. Use of the exopolysaccharide obtainable by the method according to claim 1 1 , or the exopolysaccharide according to claim 13, in the food, pharmaceutical, cosmetic and biomedical industries, preferably in the food industry as thickener, stabilizer, emulsifier, gelling agent, hydrocolloid, and/or inclusion bodies.

15. Composition comprising the exopolysaccharide obtainable by the method according to claim 1 1 , or the exopolysaccharide according to claim 13, and active substances, excipients and/or additives.