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1. WO2021204701 - RAPID DETECTION OF VIRAL INFECTION USING RT-PCR

Publication Number WO/2021/204701
Publication Date 14.10.2021
International Application No. PCT/EP2021/058720
International Filing Date 01.04.2021
IPC
C12Q 1/6844 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
C12Q 1/70 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
CPC
C12Q 1/6846
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6846Common amplification features
C12Q 1/701
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
701Specific hybridization probes
Applicants
  • BAYER AKTIENGESELLSCHAFT [DE]/[DE]
Inventors
  • GOLZ, Stefan
  • SUMMER, Holger
  • AIGNER, Silke
  • ELLINGER-ZIEGELBAUER, Heidrun
  • LEINEWEBER, Kirsten
  • MÜLLER, Thomas
Agents
  • BIP PATENTS
Priority Data
63/00725508.04.2020US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) RAPID DETECTION OF VIRAL INFECTION USING RT-PCR
(FR) DÉTECTION RAPIDE D'UNE INFECTION VIRALE PAR RT-PCR
Abstract
(EN) A lysis buffer comprising one non-ionic surfactant is provided which can be used as a one-step reagent of the preparation, storage, amplification, and/or detection of nucleic acids. Various embodiments of the lysis buffer of the invention comprise other substances that are compatible or useful in lysing cells, storing nucleic acids, amplifying nucleic acids, purifying nucleic acids, detecting nucleic acids, and/or other procedures for analysis of nucleic acids. Methods and kits based on the lysis buffer are also provided, including those for rapid lysis of cells and direct use of the resulting cell lysates in RT-PCR.
(FR) L'invention concerne un tampon de lyse comprenant un tensioactif non ionique qui peut être utilisé en tant que réactif en une étape de la préparation, du stockage, de l'amplification et/ou de la détection d'acides nucléiques. Divers modes de réalisation du tampon de lyse de l'invention comprennent d'autres substances qui sont compatibles ou utiles dans la lyse de cellules, le stockage d'acides nucléiques, l'amplification d'acides nucléiques, la purification d'acides nucléiques, la détection d'acides nucléiques, et/ou d'autres procédures pour l'analyse d'acides nucléiques. L'invention concerne également des procédés et des kits basés sur le tampon de lyse, comprenant ceux pour la lyse rapide de cellules et l'utilisation directe des lysats cellulaires obtenus en RT-PCR.
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