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1. WO2023008692 - CELL EXTRUSION SYSTEM, AND CELL EXTRUSION METHOD USING SAME

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Publication Number WO/2023/008692
Publication Date 02.02.2023
International Application No. PCT/KR2022/006147
International Filing Date 29.04.2022
IPC
C12M 1/00 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
C12M 3/00 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
CPC
C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
1Apparatus for enzymology or microbiology
C12M 23/48
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
23Constructional details, e.g. recesses, hinges
48Holding appliances; Racks; Supports
C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
3Tissue, human, animal or plant cell, or virus culture apparatus
C12M 47/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
47Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
06Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
C12M 47/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
47Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
12Purification
C12N 2509/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2509Methods for the dissociation of cells, e.g. specific use of enzymes
10Mechanical dissociation
Applicants
  • 주식회사 엠디뮨 MDIMUNE INC. [KR]/[KR]
Inventors
  • 정직한 JUNG, Jik Han
  • 김병진 KIM, Byoung Jin
  • 배신규 BAE, Shin Gyu
  • 오승욱 OH, Seung Wook
Agents
  • 김순웅 KIM, Soon Woong
Priority Data
63/227,13729.07.2021US
Publication Language Korean (ko)
Filing Language Korean (KO)
Designated States
Title
(EN) CELL EXTRUSION SYSTEM, AND CELL EXTRUSION METHOD USING SAME
(FR) SYSTÈME D'EXTRUSION CELLULAIRE, ET PROCÉDÉ D'EXTRUSION CELLULAIRE L'UTILISANT
(KO) 세포 압출 시스템 및 이를 이용한 세포 압출 방법
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Abstract
(EN) The present invention relates to a cell extrusion system and a cell extrusion method using same, the cell extrusion system comprising: a filter provided with a porous membrane and a holder which supports the membrane; a tube in which a cell suspension flows, the tube being connected to both sides of the filter such that the cell suspension flows through the membrane; and an extrusion unit provided with a pump that is connected to one side of the tube to transport the cell suspension. The present invention has the effect of making it possible to mass-produce cell-derived vesicles reliably and economically.
(FR) La présente invention concerne un système d'extrusion cellulaire et un procédé d'extrusion cellulaire l'utilisant, le système d'extrusion cellulaire comprenant les éléments suivants : un filtre muni d'une membrane poreuse et un support supportant la membrane ; un tube dans lequel s'écoule une suspension cellulaire, le tube étant relié aux deux côtés du filtre afin que la suspension cellulaire s'écoule à travers la membrane ; et une unité d'extrusion munie d'une pompe reliée à un côté du tube pour transporter la suspension cellulaire. La présente invention permet de produire en masse des vésicules dérivées de cellules de manière fiable et économique.
(KO) 본 발명은 세포 압출 시스템 및 이를 이용한 세포 압출 방법에 관한 것으로, 더욱 상세하게는 다공성 멤브레인 및 상기 멤브레인을 지지하는 홀더를 구비하는 필터; 내부로 세포부유액이 흐르도록 구비되고, 상기 세포부유액이 상기 멤브레인을 통과하여 흐르도록 상기 필터의 양측에 연결되는 튜브; 및 상기 튜브의 일측에 연결되어 상기 세포부유액을 이송시키는 펌프를 구비하는 압출유닛을 포함하는 세포 압출 시스템과 이를 이용한 세포 압출 방법에 관한 것이다. 이러한 본 발명은 세포 유래 베시클을 안정적이면서도 경제적이고, 대량으로 제조할 수 있는 효과가 있다.
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