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1. WO1992014448 - BINDING OF GROWTH HORMONE TO LIPOSOMES

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[ EN ]

CLAIMS

What I claim is:

1. A process for producing modified liposomes comprising the steps of:
(a) providing a reaction vessel containing a liposome
component having liposome and phosphatidylethanolamine;

5 (b) adding a recognizing substance component of a
growth factor to the reaction vessel ;

(c) buffering the reaction mixture of the liposome and recognizing substance components;

(d) adding a crosslinking reagent to the buffered ° reaction mixture; and

(e) allowing the buffered reaction mixture with
reagent to incubate for a period of time sufficient
for the modified liposome to form.

2. The process of claim 1 wherein the liposome is
selected from the group consisting of multilamellar vesicles, microemulsified liposomes and large unilamellar vesicles.

3. The process of claim 1 wherein the growth factor is epidermal growth factor.

4. The process of claim 1 wherein the growth factor is urogasterone.

5. The process of claim 1 wherein the crosslinking reagent is glutaraldehyde.

6. The process of claim 1 wherein the crosslinking reagent is a bifunctional oxirane.

7. The process of claim 1 where in the crosslinking reagent is ethylene glycol diglycidyl ether.

8. The process of claim 1 wherein the recognizing substance is labeled with a fluorescent marker.

9. The process of claim 1 wherein the recognizing substance is labeled with a iodinated marker.

10. A modified liposome produced according to the method of claim 1.

11. A process for covalently bonding a recognizing
substance to a liposome comprising the steps of:

(a) providing a reaction vessel containing a liposome
component having liposome and phosphatidylethanolamine;

5 (b) adding a recognizing substance component of growth factor to the reaction vessel;

(c) buffering the reaction mixture of the liposome and recognizing substance components;

(d) adding a crosslinking reagent to the buffered 10 reaction mixture;

(e) allowing the buffered reaction mixture with
reagent to incubate for a period of time sufficient
for the covalent bondi g of amine residues of the
recognizing substance and iposome components; and

I5 (f) separation and removal of covalently bound
recognizing substance liposome components from excess
unreacted and noncovalently bound materials.

12. Tne process of claim 11 wherein the liposome is
selected from the group consisting of multilamellar vesicles, microemulsified liposomes and large unilamellar vesicles.

13. The process of claim 11 wherein the growth factor is epidermal growth factor.

14. The process of claim 11 wherein the growth factor is urogasterone.

15. The process of claim 11 wherein the crosslinking reagent is glutaraldehyde.

16. The process of claim 11 wherein the crosslinking reagent is a bifunctional oxirane.

17. The process of claim 11 where in the crosslinking reagent is ethylene glycol diglycidyl ether.

18. The process of claim 11 wherein the recognizing substance component is labeled with a fluorescent marker.

19. The process of claim 11 wherein the recognizing substance component is labeled with a iodinated marker.

20. A modified liposome produced according to the method of claim 11.

21. A modified liposome comprising a liposome component covalently linked to a recognizing substance component by a crosslinking reagent.

22. The modified liposome of claim 21 wherein the liposome component includes phosphatidylethanol amine.

23. The modified liposome of claim 21 wherein the liposome component is selected from the group consisting of
multilamellar vesicles, microemulsified liposomes and large unilamellar vesicles.

24. The modified liposome of claim 21 wherein the recognizing substance component is a growth factor.

25. The modified liposome of claim 21 wherein the recognizing substance component is an epidermal growth factor.

26. The modified liposome of claim 21 wherein the recognizing substance component is urogasterone.

27. The modified liposome of claim 21 wherein the crosslinking reagent is glutaraldehyde.

28. The modified liposome of claim 21 wherein the crosslinking reagent is a bifunctional oxirane.

29. The modified liposome of claim 21 wherein the crosslinking reagent is ethylene glycol diglycidyl ether.

30. The modified liposome of claim 21 wherein the recognizing substance component is labeled with a fluorescent marker.

31. The modified liposome of claim 21 wherein the recognizing substance component is labeled with a iodinated marker.

32. The modified liposome of claim 21 wherein the crosslinking reagent is selected from a group of reagents linking amine residues of the liposome component and the recognizing substance component.