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1. EP2735613 - TECHNIQUE FOR CLEAVING OUT PART OF poly(A) CHAIN AND/OR 3'-TERMINAL SEQUENCE OF mRNA TO INHIBIT TRANSLATION REACTION

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[ EN ]
Claims

1. A method of inhibiting the translation reaction of a target gene, comprising cutting out a part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA.
  2. The method according to claim 1, wherein a part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA is cut out with a ribonucleic acid which is capable of hybridizing to the part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA.
  3. The method according to claim 2, wherein the ribonucleic acid comprises a nucleotide sequence complementary to the whole or a part of a sequence encoding the region from the poly(A) tail junction of the target mRNA to the 40 th nucleotide upstream therefrom.
  4. The method according to claim 3, wherein the whole or a part of a sequence encoding the region of 40 nucleotides from the poly(A) tail junction of the target mRNA to the 40 th nucleotide upstream therefrom contains the whole or a part of a polyadenylation signal sequence.
  5. The method according to claim 3 or 4, wherein the ribonucleic acid is a 20- to 25-mer.
  6. The method according to any one of claims 2 to 5, wherein the ribonucleic acid comprises natural nucleotides.
  7. The method according to claim 6, wherein the ribonucleic acid is a double-stranded RNA.
  8. The method according to any one of claims 2 to 5, wherein the ribonucleic acid contains at least one nucleotide analogue.
  9. The method according to claim 8, wherein the ribonucleic acid is single-stranded.
  10. The method according to claim 9, wherein the single-stranded ribonucleic acid is an antisense morpholino oligonucleotide.
  11. A kit for inhibiting the translation reaction of a target gene, comprising a reagent capable of cutting out a part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA.
  12. The kit according to claim 11, wherein the reagent capable of cutting out a part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA is a ribonucleic acid.
  13. A cell in which the translation reaction of a target gene is inhibited by introduction thereinto of a reagent capable of cutting out a part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA.
  14. A non-human organism in which the translation reaction of a target gene is inhibited by introduction thereinto of a reagent capable of cutting out a part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA.