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1. US20190335800 - Hemp Protein and Use for Microencapsulation

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[ EN ]

Claims

1. A microencapsulated product of microcapsules, wherein the microcapsules comprise:
(a) a core comprising a lipid based component; and
(b) a coating comprising a hemp seed protein fraction obtained by alkaline aqueous extraction from a hemp seed protein source such that the hemp protein fraction is water soluble under alkaline conditions and is capable of forming a stable emulsion with the lipid based component.
2. The microencapsulated product of claim 1, wherein the hemp protein fraction is water soluble at a pH range of 8.5 to 11.5.
3. The microencapsulated product of claim 1, wherein the hemp protein fraction is water soluble at a pH range of 9 to 11.
4. The microencapsulated product of claim 1, wherein the hemp protein fraction has a molecular weight in the range of 5,000-500,000 Da.
5. The microencapsulated product of claim 4, wherein the hemp protein fraction has a molecular weight in the range of 10,000-500,000 Da.
6. The microencapsulated product of claim 1, wherein the hemp protein fraction for the coating is obtained by precipitating from an aqueous solution of the hemp seed protein source at or above an isoelectric pH of the hemp protein and at a temperature less than 70° C., or by ultrafiltration from an aqueous solution of the hemp seed protein source.
7. The microencapsulated product of claim 6, wherein the hemp protein fraction for the coating is obtained by precipitating from an aqueous solution of the hemp seed protein source at a pH in the range of 4.5-5.5, and at a temperature not greater than 60° C.
8. The microencapsulated product of claim 1, wherein the hemp protein fraction for the coating is modified by enzyme hydrolysis or succinylation.
9. The microencapsulated product of claim 1, wherein the coating further includes one or more carbohydrates selected from starch, modified starch, polysaccharides, maltodextrin, fibre, hydrocollids, gum arabic, gelan gum, carageenan, alginates, and lactates.
10. The microencapsulated product of claim 1, wherein the lipid based component is an edible oil, an essential oil, a wax or a flavour.
11. The microencapsulated product of claim 10, wherein the edible oil is derived from one or more of an oilseed crop, cereal crop, legume, algae, fish and yeast.
12. The microencapsulated product of claim 11, wherein the edible oil is derived from one or more of hemp seed, canola, flax, microalgal, soybean, oat, chickpea, camelina, coconut and fish.
13. The microencapsulated product of claim 1, wherein the lipid based component comprises greater than 30% by weight of the microencapsulated product in a dry powder form.
14. The microencapsulated product of claim 13, wherein the lipid based component comprises in the range of 35-75% weight of the microencapsulated product in a dry powder form.
15. A method of extracting a hemp protein fraction comprising:
a) providing a hemp seed protein source;
b) contacting the hemp seed protein source with an alkaline aqueous solution to extract hemp protein from the hemp seed protein source into an aqueous hemp protein solution;
c) separating the aqueous hemp protein solution from residual hemp protein source solids; and
d) isolating a hemp protein fraction by either:
i. precipitating hemp proteins from the separated aqueous hemp protein solution at or above an isoelectric pH of the hemp protein and at a temperature less than 70° C., and separating the precipitated proteins to produce the hemp seed protein fraction; or
ii. ultrafiltering the separated aqueous hemp protein solution to produce the hemp seed protein fraction having a molecular weight of 5,000 Da and higher.
16. The method of claim 15, wherein the pH of the extraction in step b) is in the range of 8.5-11.5.
17. The method of claim 16, wherein the hemp protein fraction is produced by step d, i) at a pH in the range of 4.5-5.5.
18. The method of claim 16, wherein the hemp protein fraction is produced by step d, ii).
19. The method of claim 16, wherein:
the hemp seed protein source is one or more of a defatted or non-defatted hemp seed, a hemp hull, and a defatted or non-defatted dehulled hemp meal;
the hemp seed protein source is optionally milled to a fine powder which is optionally screened prior to extraction; and
the hemp seed protein source is selected from the group consisting of Cannabis sativa, Cannabis indica, and Cannabis ruderalis, or the hemp seed protein source is from a variety of Cannabis sativa.
20. The method of claim 16, wherein the alkaline aqueous solution in step b) has a pH in the range of 9-11, and wherein the temperature during steps a), b) and c) is less than 70° C.
21. The method of claim 20, wherein the hemp protein fraction is produced by step d ii), and wherein the ultrafiltration is conducted with a selective membrane for 10,000 Da and higher, at a pH in the range of 9-11, and at a temperature during steps a), b) and c) is less than 70° C.
22. The method of claim 16, wherein the aqueous hemp protein solution is separated from the residual hemp protein source solids by centrifuging.
23. The method of claim 16, wherein the hemp protein fraction is further treated by one or more steps including diafiltration, washing, pasteurization, drying and spray drying.
24. The method of claim 1, further comprising:
e) forming a stable emulsion of the hemp seed protein fraction with a lipid based component.
25. The method of claim 24, further comprising:
f) drying the stable emulsion to form a microencapsulated product of microcapsules having a core comprising the lipid based component and a coating comprising the hemp seed protein fraction.
26. A hemp protein fraction for use in microencapsulating a lipid based component, wherein the hemp protein fraction is obtained by alkaline aqueous extraction from a hemp seed protein source such that the hemp protein is water soluble under alkaline conditions and is capable of forming a stable emulsion with the lipid based component.