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1. KR1020210125536 - 접선 유동 여과에 의한 mRNA 정제

Office
Republic of Korea
Application Number 1020217028845
Application Date 11.02.2020
Publication Number 1020210125536
Publication Date 18.10.2021
Publication Kind A
IPC
C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
A61K 31/7088
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31Medicinal preparations containing organic active ingredients
70Carbohydrates; Sugars; Derivatives thereof
7088Compounds having three or more nucleosides or nucleotides
CPC
C12N 15/1017
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1017by filtration, e.g. using filters, frits, membranes
A61K 31/7088
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
31Medicinal preparations containing organic active ingredients
70Carbohydrates; Sugars; Derivatives thereof
7088Compounds having three or more nucleosides or nucleotides
Applicants 에트리스 게엠베하
Inventors 가이거, 요하네스
트레믈, 마틴
Agents 양영준
김영
Priority Data 19156522.5 11.02.2019 EP
Title
(KO) 접선 유동 여과에 의한 mRNA 정제
Abstract
(KO) 본 개시내용은 mRNA 분자를 정제하는 방법으로서, (Ia) 침전된 mRNA 분자를 포함하는 현탁액으로부터 침전된 mRNA 분자를 정제하는 단계, (Ib) 정제된 침전된 mRNA 분자를 세척하고 용해시키는 단계, (IIa) 킬레이트제를 포함하는 용액을 사용하여 mRNA 분자를 정제하는 단계, 이어서 (IIb) 정제된 mRNA 분자를 세척하는 단계를 포함하며, 여기서 단계 (Ia) 내지 (IIb)는 접선 유동 여과를 사용하여 수행되는 것인 방법을 제공한다.
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