(12) International Application Status Report

Received at International Bureau: 13 December 2019 (13.12.2019)

Information valid as of: 15 April 2020 (15.04.2020)

Report generated on: 27 September 2020 (27.09.2020)

(10) Publication number: (43) Publication date: (26) Publication language:
WO 2020/11296404 June 2020 (04.06.2020) English (EN)

(21) Application number: (22) Filing date: (25) Filing language:
PCT/US2019/06355127 November 2019 (27.11.2019) English (EN)

(31) Priority number(s): (32) Priority date(s): (33) Priority status:
62/772,979 (US)29 November 2018 (29.11.2018) Priority document received (in compliance with PCT Rule 17.1)

(51) International Patent Classification:
C12Q 1/6876 (2018.01); C12Q 1/6874 (2018.01); G16B 30/20 (2019.01)

(71) Applicant(s):
XGENOMES CORP. [US/US]; The Engine 501 Massachusetts Ave Cambridge, MA 02139 (US) (for all designated states)

(72) Inventor(s):
MIR, Kalim; The Engine 501 Massachusetts Ave Cambridge, MA 02139 (US)

(74) Agent(s):
LOVEJOY, Brett, A.; Morgan, Lewis & Bockius LLP One Market, Spear Street Tower San Francisco, CA 94105 (US)

(54) Title (EN): SEQUENCING BY COALASCENCE
(54) Title (FR): SÉQUENCAGE PAR COALESCENCE

(57) Abstract:
(EN): A method of sequencing a single, elongated target polynucleotide molecule can include the steps of seeding a plurality of separately resolvable origins of polynucleotide synthesis along the single, elongated target polynucleotide; contacting the target polynucleotide with a polymerase and labelled nucleotides; incorporating a labelled nucleotide, using the polymerase, into a plurality of sequence fragments complementary to the target polynucleotide and originating from the origins of polynucleotide synthesis; identifying and storing the identity and positions of the labelled nucleotide incorporated into each of the plurality of sequence fragments; and repeating the incorporating and identifying steps until adjacent sequence fragments coalesce and result in continuous sequence reads spanning two or more adjacent sequence fragments.
(FR): Un procédé de séquençage d'une seule molécule polynucléotidique cible allongée peut consister à ensemencer une pluralité d'origines de synthèse de polynucléotides pouvant être résolues séparément, le long du polynucléotide cible allongé unique; à mettre en contact le polynucléotide cible avec une polymérase et des nucléotides marqués; à incorporer un nucléotide marqué, à l'aide de la polymérase, en une pluralité de fragments de séquence complémentaires du polynucléotide cible et provenant des origines de synthèse de polynucléotides; à identifier et stocker l'identité et les positions du nucléotide marqué incorporé dans chaque fragment de la pluralité de fragments de séquence; et à répéter les étapes d'incorporation et d'identification jusqu'à ce que des fragments de séquence adjacents coalescent et conduisent à des lectures de séquence continue couvrant au moins deux fragments de séquence adjacents.

International search report:
Received at International Bureau: 16 March 2020 (16.03.2020) [IL]

International Report on Patentability (IPRP) Chapter II of the PCT:
Not available

(81) Designated States:
AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
European Patent Office (EPO) : AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR
African Intellectual Property Organization (OAPI) : BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG
African Regional Intellectual Property Organization (ARIPO) : BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW
Eurasian Patent Organization (EAPO) : AM, AZ, BY, KG, KZ, RU, TJ, TM