(12) International Application Status Report

Received at International Bureau: 03 September 2018 (03.09.2018)

Information valid as of: 07 December 2018 (07.12.2018)

Report generated on: 17 August 2019 (17.08.2019)

(10) Publication number: (43) Publication date: (26) Publication language:
WO 2019/04564907 March 2019 (07.03.2019) English (EN)

(21) Application number: (22) Filing date: (25) Filing language:
PCT/SG2018/05043829 August 2018 (29.08.2018) English (EN)

(31) Priority number(s): (32) Priority date(s): (33) Priority status:
10201707052R (SG)29 August 2017 (29.08.2017) Priority document received (in compliance with PCT Rule 17.1)

(51) International Patent Classification:
C12N 5/0775 (2010.01)

(71) Applicant(s):
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way #20-10 Connexis North Tower Singapore 138632 (SG) (for all designated states)

(72) Inventor(s):
LIAU, Brian; c/o Bioprocessing Technology Institute, 20 Biopolis Way Centros, #03-01 Singapore 138668 (SG)
RAGHOTHAMAN, Deepak; c/o Bioprocessing Technology Institute, 20 Biopolis Way, Centros, #03-01, Singapore 138668 (SG)
OH, Steve; c/o Bioprocessing Technology Institute, 20 Biopolis Way Centros, #03-01, Singapore 138668 (SG)

(74) Agent(s):
SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531 Robinson Road Post Office Singapore 903031 (SG)

(54) Title (EN): METHODS FOR ENRICHING MESENCHYMAL STEM CELLS
(54) Title (FR): MÉTHODES POUR ENRICHIR DES CELLULES SOUCHES MÉSENCHYMATEUSES

(57) Abstract:
(EN): Methods of enriching non-senescent mesenchymal stem cells (MSCs) and methods of performing quality control for non-senescent MSCs are disclosed. The methods comprise contacting a sample comprising MSCs with a probe (specifically peanut agglutinin, PNA) that binds to a glycan (specifically glycan T-antigen) expressed on the surface of MSCs, with the separation or determination of non-senescent MSCs from senescent MSCs based on a difference in expression of the glycan at the cell surface, i.e. the non-senescent MSCs express more of the glycan than senescent MSCs. A high amount of non-senescent MSCs is indicative of a sample of acceptable quality.
(FR): L'invention concerne des méthodes pour enrichir des cellules souches mésenchymateuses (MSC) non sénescentes, ainsi que des méthodes permettant de réaliser un contrôle qualité portant sur les MSC non sénescentes. Les méthodes comprennent la mise en contact d'un échantillon comprenant des MSC avec une sonde (spécifiquement l'agglutinine d'arachide, PNA) qui se lie à un glycane (spécifiquement le glycane antigène T) exprimé en surface des MSC, la séparation ou la détermination des MSC non sénescentes des MSC sénescentes se fondant sur la différence d'expression du glycane au niveau de la surface cellulaire, les MSC non sénescentes exprimant une plus grande quantité du glycane que les MSC sénescentes. Une quantité élevée de MSC non sénescentes indique un échantillon de qualité acceptable.

International search report:
Received at International Bureau: 05 November 2018 (05.11.2018) [SG]

International Report on Patentability (IPRP) Chapter II of the PCT:
Not available

(81) Designated States:
AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
European Patent Office (EPO) : AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR
African Intellectual Property Organization (OAPI) : BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG
African Regional Intellectual Property Organization (ARIPO) : BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW
Eurasian Patent Organization (EAPO) : AM, AZ, BY, KG, KZ, RU, TJ, TM

Declarations:
Declaration of inventorship (Rules 4.17(iv) and 51bis.1(a)(iv)) for the purposes of the designation of the United States of America