(12) International Application Status Report

Received at International Bureau: 27 August 2018 (27.08.2018)

Information valid as of: 22 January 2019 (22.01.2019)

Report generated on: 20 September 2019 (20.09.2019)

(10) Publication number: (43) Publication date: (26) Publication language:
WO 2019/03309514 February 2019 (14.02.2019) English (EN)

(21) Application number: (22) Filing date: (25) Filing language:
PCT/US2018/04647713 August 2018 (13.08.2018) English (EN)

(31) Priority number(s): (32) Priority date(s): (33) Priority status:
62/544,228 (US)11 August 2017 (11.08.2017)

(51) International Patent Classification:
C07K 14/195 (2006.01); C12N 9/12 (2006.01); C12P 21/00 (2006.01)

(71) Applicant(s):
SYNVITROBIO, INC. [US/US]; 953 Indiana Street San Francisco, California 94107 (US) (for all designated states)

(72) Inventor(s):
SUN, Zachary Z.; c/o Synvitrobio, Inc. 953 Indiana Street San Francisco, California 94107 (US)
CHIAO, Abel C.; c/o Synvitrobio, Inc. 953 Indiana Street San Francisco, California 94107 (US)
ROBERTSON, Dan E.; c/o Synvitrobio, Inc. 953 Indiana Street San Francisco, California 94107 (US)
METZGER IV, Louis E.; c/o Synvitrobio, Inc. 953 Indiana Street San Francisco, California 94107 (US)
MANSFIELD, Richard; c/o Synvitrobio, Inc. 953 Indiana Street San Francisco, California 94107 (US)
TREGO, Kelly S.; c/o Synvitrobio, Inc. 953 Indiana Street San Francisco, California 94107 (US)

(74) Agent(s):
XIE, Fang; Greenberg Traurig LLP One International Place Suite 2000 Boston, Massachusetts 02110 (US)

(54) Title (EN): IMPROVED IN VITRO TRANSCRIPTION/TRANSLATION (TXTL) SYSTEM AND USE THEREOF
(54) Title (FR): SYSTÈME AMÉLIORÉ DE TRANSCRIPTION/TRADUCTION (TXTL) IN VITRO ET SON UTILISATION

(57) Abstract:
(EN): Provided herein, in one aspect, is a composition for in vitro transcription and translation, comprising: a treated cell lysate derived from one or more host cells such as bacteria, archaea, plant or animal; a plurality of supplements for gene transcription and translation; an energy recycling system for providing and recycling adenosine triphosphate (ATP); and one or more exogenous additives. Methods for making and using the same are also provided.
(FR): Selon un aspect, l'invention concerne une composition pour la transcription et la traduction in vitro, comprenant : un lysat cellulaire traité dérivé d'un ou de plusieurs cellules hôtes telles que des bactéries, des archées, une plante ou un animal ; une pluralité de suppléments pour la transcription et la traduction géniques ; un système de recyclage d'énergie pour fournir de l'adénosine triphosphate (ATP) ; et un ou plusieurs additifs exogènes. L'invention concerne également des procédés de fabrication et d'utilisation de ladite composition.

International search report:
Received at International Bureau: 15 December 2018 (15.12.2018) [US]

International Report on Patentability (IPRP) Chapter II of the PCT:
Not available

(81) Designated States:
AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
European Patent Office (EPO) : AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR
African Intellectual Property Organization (OAPI) : BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG
African Regional Intellectual Property Organization (ARIPO) : BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW
Eurasian Patent Organization (EAPO) : AM, AZ, BY, KG, KZ, RU, TJ, TM