(12) International Application Status Report

Received at International Bureau: 08 August 2018 (08.08.2018)

Information valid as of: 29 October 2018 (29.10.2018)

Report generated on: 19 April 2019 (19.04.2019)

(10) Publication number: (43) Publication date: (26) Publication language:
WO 2019/02608207 February 2019 (07.02.2019) English (EN)

(21) Application number: (22) Filing date: (25) Filing language:
PCT/IL2018/05086302 August 2018 (02.08.2018) English (EN)

(31) Priority number(s): (32) Priority date(s): (33) Priority status:
253801 (IL)02 August 2017 (02.08.2017) Priority document received (in compliance with PCT Rule 17.1)

(51) International Patent Classification:
C07K 19/00 (2006.01); C12N 15/62 (2006.01); C12Q 1/32 (2006.01); G01N 33/66 (2006.01)

(71) Applicant(s):
B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY [IL/IL]; P.O. Box 653 84105 Beer Sheva (IL) (for all designated states)

(72) Inventor(s):
ALFONTA, Lital; 54 Nurit Street 8496500 Omer (IL)
ZARIVACH, Raz; 8 Bentwich Street 8486621 Beer Sheva (IL)
GRUSHKA, Jennifer; 10 HaShla Sreet 6228312 Tel Avv (IL)
ALGOV, Itay; 19 HaAlon Street 6092000 Kadima (IL)

(74) Agent(s):
GEYRA, Assaf; Geyra Gassner Kesten 55 Yigal Alon Street 6789115 Tel Aviv (IL)

(54) Title (EN): A RECOMBINANT FLAVIN-ADENINE DINUCLEOTIDE GLUCOSE DEHYDROGENASE AND USES THEREOF
(54) Title (FR): FLAVINE ADÉNINE DINUCLÉOTIDE DE GLUCOSE DÉSHYDROGÉNASE RECOMBINANTE ET UTILISATIONS ASSOCIÉES

(57) Abstract:
(EN): A recombinant protein, including: (a) alpha subunit of an FAD-GDH; and (b) a minimal cytochrome c peptide is provided. Additionally, an electrode coupled to a recombinant protein, the recombinant protein made of: (a) a cofactor of a redox enzyme; (b) a redox enzyme; (c) a linker moiety configured to link any one of: the cofactor or the enzyme to an electron transfer (ET) domain; and (d) an ET domain, is also provided. Methods for: (a) transferring an electron to an electrode, by coupling the recombinant protein an electrode; and (b) quantifying the amount of an analyte e.g., glucose are also provided.
(FR): L'invention concerne une protéine recombinante, comprenant : (a) une sous-unité alpha d'une FAD-GDH ; et (b) un peptide de cytochrome c minimal. L'invention concerne également une électrode couplée à une protéine recombinante, la protéine recombinante étant constituée de : (a) un cofacteur d'une enzyme redox ; (b) une enzyme redox ; (c) une fraction de liaison configurée pour lier l'un quelconque des éléments suivants : le cofacteur ou l'enzyme à un domaine de transfert d'électrons (ET) ; ET (d) un domaine ET. L'invention concerne en outre des procédés de : (a) transfert d'un électron vers une électrode, par couplage de la protéine recombinante à une électrode ; et (b) quantification de la quantité d'un analyte, par exemple, du glucose.

International search report:
Received at International Bureau: 28 October 2018 (28.10.2018) [IL]

International Report on Patentability (IPRP) Chapter II of the PCT:
Not available

(81) Designated States:
AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
European Patent Office (EPO) : AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR
African Intellectual Property Organization (OAPI) : BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG
African Regional Intellectual Property Organization (ARIPO) : BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW
Eurasian Patent Organization (EAPO) : AM, AZ, BY, KG, KZ, RU, TJ, TM