(12) International Application Status Report

Received at International Bureau: 01 February 2010 (01.02.2010)

Information valid as of: 07 July 2010 (07.07.2010)

Report generated on: 14 December 2019 (14.12.2019)

(10) Publication number: (43) Publication date: (26) Publication language:
WO 2010/08417129 July 2010 (29.07.2010) English (EN)

(21) Application number: (22) Filing date: (25) Filing language:
PCT/EP2010/05072322 January 2010 (22.01.2010) English (EN)

(31) Priority number(s): (32) Priority date(s): (33) Priority status:
61/146,449 (US)22 January 2009 (22.01.2009) Priority document received (in compliance with PCT Rule 17.1)

(51) International Patent Classification:
C07K 14/72 (2006.01); C12N 9/00 (2006.01); C12N 15/90 (2006.01); C12N 9/90 (2006.01)

(71) Applicant(s):
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) [FR/FR]; 3, rue Michel Ange F-75016 Paris (FR) (for all designated states except US)
THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS [US/US]; 352 Henry Administration Building MC350 506.S. Wright Street Urbana, Illinois 61801-3642 (US) (for all designated states except US)
UNIVERSITE DE STRASBOURG [FR/FR]; 4 rue Blaise Pascal F-67000 Strasbourg (FR) (for all designated states except US)
METZGER, Daniel [FR/FR]; 26A, rue de l'Oberelsau F-67200 Strasbourg (FR) (for US only)
CHAMBON, Pierre [FR/FR]; 4, rue du Dr. Albert Scheitzer F-67113 Blaesheim (FR) (for US only)
ZHAO, Huimin [US/US]; 2109 Scottsdale Drive Champaign, Illinois 61801 (US) (for US only)
KATZENELLENBOGEN, John [US/US]; 704 West Pennsylvania Avenue Urbana, Illinois 61801 (US) (for US only)

(72) Inventor(s):
METZGER, Daniel; 26A, rue de l'Oberelsau F-67200 Strasbourg (FR)
CHAMBON, Pierre; 4, rue du Dr. Albert Scheitzer F-67113 Blaesheim (FR)
ZHAO, Huimin; 2109 Scottsdale Drive Champaign, Illinois 61801 (US)
KATZENELLENBOGEN, John; 704 West Pennsylvania Avenue Urbana, Illinois 61801 (US)

(74) Agent(s):
WARCOIN, Jacques; Cabinet Regimbeau 20, rue de Chazelles F-75847 Paris Cedex 17 (FR)

(54) Title (EN): METHOD FOR TEMPORALLY CONTROLLING THE BIOLOGICAL ACTIVITY OF PROTEINS IN VERTEBRATES, AND APPLICATIONS THEREOF
(54) Title (FR): PROCÉDÉ POUR CONTRÔLER TEMPORAIREMENT L'ACTIVITÉ BIOLOGIQUE DE PROTÉINES CHEZ LES VERTÉBRÉS, ET SES APPLICATIONS

(57) Abstract:
(EN): The present invention relates to a method for tightly temporally controlling the biological activity of a protein of interest in a vertebrate, upon induction of the activity of a fusion protein comprising said protein of interest and an ERM polypeptide containing a mutated ligand binding domain of the human oestrogen receptor α, with a synthetic ligand that does not interfere with oestrogen signalling. In particular, the present invention concerns a method for generating tightly temporally-controlled targeted somatic mutations in a vertebrate, preferably a mouse, by inducing the activity of a fusion protein comprising a site- specific recombinase protein and an ERM polypeptide, with a synthetic ligand devoid of oestrogenic and anti-oestrogenic activities.
(FR): La présente invention concerne un procédé pour un contrôle temporaire ferme de l'activité biologique d'une protéine intéressante chez un vertébré, lors de l'induction de l'activité d'une protéine de fusion comprenant ladite protéine intéressante et un polypeptide ERM contenant un domaine de liaison au ligand muté du récepteur α des œstrogènes humains, celui-ci étant un ligand synthétique qui n'interfère pas avec la signalisation des œstrogènes. En particulier, la présente invention concerne un procédé pour générer des mutations somatiques ciblées avec un contrôle temporaire ferme chez un vertébré, de préférence une souris, en induisant l'activité d'une protéine de fusion comprenant une protéine recombinase régio-spécifique et un polypeptide ERM, avec un ligand synthétique dépourvu des activités œstrogénique et anti-œstrogénique.

International search report:
Received at International Bureau: 12 April 2010 (12.04.2010) [EP]

International Report on Patentability (IPRP) Chapter II of the PCT:
Not available

(81) Designated States:
AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
European Patent Office (EPO) : AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR
African Intellectual Property Organization (OAPI) : BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG
African Regional Intellectual Property Organization (ARIPO) : BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW
Eurasian Patent Organization (EAPO) : AM, AZ, BY, KG, KZ, MD, RU, TJ, TM

Declarations:
Declaration made as applicant's entitlement, as at the international filing date, to apply for and be granted a patent (Rules 4.17(ii) and 51bis.1(a)(ii)), in a case where the declaration under Rule 4.17(iv) is not appropriate
Declaration made as applicant's entitlement, as at the international filing date, to claim the priority of the earlier application, where the applicant is not the applicant who filed the earlier application or where the applicant's name has changed since the filing of the earlier application (Rules 4.17(iii) and 51bis.1(a)(iii))