(12) International Application Status Report

Received at International Bureau: 07 January 2009 (07.01.2009)

Information valid as of: 12 July 2010 (12.07.2010)

Report generated on: 03 March 2021 (03.03.2021)

(10) Publication number: (43) Publication date: (26) Publication language:
WO 2009/07773425 June 2009 (25.06.2009) English (EN)

(21) Application number: (22) Filing date: (25) Filing language:
PCT/GB2008/00412715 December 2008 (15.12.2008) English (EN)

(31) Priority number(s): (32) Priority date(s): (33) Priority status:
0724736.4 (GB)19 December 2007 (19.12.2007) Priority document received (in compliance with PCT Rule 17.1)
61/080,492 (US)14 July 2008 (14.07.2008) Priority document received (in compliance with PCT Rule 17.1)

(51) International Patent Classification:
G01N 33/487 (2006.01); B01D 67/00 (2006.01)

(71) Applicant(s):
OXFORD NANOPORE TECHNOLOGIES LIMITED [GB/GB]; Edmund Cartwright House 4 Robert Robinson Avenue Oxford Science Park Oxford OX4 4GA (GB) (for all designated states except US)
REID, Stuart, William [GB/GB]; 135 Queens Road Vicars Cross Chester CH3 5HF (GB) (for US only)
REID, Terence, Alan [GB/GB]; Priory Barn Priory Lane Bicester OX26 6BG (GB) (for US only)
CLARKE, James, Anthony [GB/GB]; Holly Farm Layer Breton Colchester CO2 0PS (GB) (for US only)
WHITE, Steven, Paul [GB/GB]; 40 Hamilton Road Oxford OX2 7PZ (GB) (for US only)
SANGHERA, Gurdial, Singh [GB/GB]; 23 Five Mile Drive Oxford OX2 8HT (GB) (for US only)

(72) Inventor(s):
REID, Stuart, William; 135 Queens Road Vicars Cross Chester CH3 5HF (GB)
REID, Terence, Alan; Priory Barn Priory Lane Bicester OX26 6BG (GB)
CLARKE, James, Anthony; Holly Farm Layer Breton Colchester CO2 0PS (GB)
WHITE, Steven, Paul; 40 Hamilton Road Oxford OX2 7PZ (GB)
SANGHERA, Gurdial, Singh; 23 Five Mile Drive Oxford OX2 8HT (GB)

(74) Agent(s):
MERRYWEATHER, Colin, Henry; J.A. Kemp & Co. 14 South Square Gray's Inn London WC1R 5LX (GB)

(54) Title (EN): FORMATION OF LAYERS OF AMPHIPHILIC MOLECULES
(54) Title (FR): FORMATION DE COUCHES DE MOLÉCULES AMPHIPHILES

(57) Abstract:
(EN): To form a layer (11) separating two volumes of aqueous solution, especially a biolayer lipid membrane (BLM) there is used an apparatus comprising elements defining a chamber (7), the elements including a body (2) of non-conductive material having formed therein at least one recess (5) opening into the chamber, the recess containing an electrode (21). A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.
(FR): Pour former une couche séparant deux volumes de solution aqueuse, on utilise un appareil comprenant des éléments délimitant une chambre, les éléments comprenant un corps en matière non conductrice ayant, formée dans celui-ci, au moins une cavité s'ouvrant dans la chambre, la cavité contenant une électrode. Un revêtement de prétraitement constitué d'un fluide hydrophobe est appliqué sur le corps dans toute la cavité. On fait couler une solution aqueuse, ayant des molécules amphiphiles ajoutées à celle-ci, d'un côté à l'autre du corps pour recouvrir la cavité de sorte que la solution aqueuse est introduite dans la cavité à partir de la chambre et qu'une couche des molécules amphiphiles se forme dans toute la cavité séparant un volume de solution aqueuse introduit dans la cavité du volume restant de solution aqueuse.

International search report:
Received at International Bureau: 11 August 2009 (11.08.2009) [EP]

International Report on Patentability (IPRP) Chapter II of the PCT:
Not available

(81) Designated States:
AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
European Patent Office (EPO) : AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR
African Intellectual Property Organization (OAPI) : BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG
African Regional Intellectual Property Organization (ARIPO) : BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW
Eurasian Patent Organization (EAPO) : AM, AZ, BY, KG, KZ, MD, RU, TJ, TM