(12) International Application Status Report

Received at International Bureau: 06 March 2006 (06.03.2006)

Information valid as of: Not available

Report generated on: 25 July 2021 (25.07.2021)

(10) Publication number: (43) Publication date: (26) Publication language:
WO 2006/05583626 May 2006 (26.05.2006) English (EN)

(21) Application number: (22) Filing date: (25) Filing language:
PCT/US2005/04195316 November 2005 (16.11.2005) English (EN)

(31) Priority number(s): (32) Priority date(s): (33) Priority status:
60/628,211 (US)16 November 2004 (16.11.2004) Priority document received (in compliance with PCT Rule 17.1)

(51) International Patent Classification:
C12N 15/74 (2006.01)

(71) Applicant(s):
PRESIDENT AND FELLOWS OF HARVARD COLLEGE [US/US]; 17 Quincy Street Cambridge, MA 02138 (US) (for all designated states except US)
CHURCH, George, M. [US/US]; 218 Kent Street Brookline, MA 02446 (US) (for US only)
REPPAS, Nikos [US/US]; 13 Bartlett Crescent #2 Brookline, MA 02446 (US) (for US only)

(72) Inventor(s):
CHURCH, George, M.; 218 Kent Street Brookline, MA 02446 (US)
REPPAS, Nikos; 13 Bartlett Crescent #2 Brookline, MA 02446 (US)

(74) Agent(s):
IWANICKI, John, P.; Banner & Witcoff, Ltd. 28 State Street, 28th Floor Boston, MA 02109 (US)

(54) Title (EN): IN VIVO ALTERATION OF CELLULAR DNA
(54) Title (FR): ALTERATION IN VIVO DE L'ADN CELLULAIRE

(57) Abstract:
(EN): The present invention relates to methods, cells and compositions for performing in vivo recombination without the need for clonal selection. These methods, cells and compositions are useful for the automation of recombination. The present invention provides methods for performing sequential steps in genome engineering, as well as for achieving parallel and multiplex genome engineering.
(FR): La présente invention concerne des méthodes, des cellules et des compositions destinées à la réalisation d'une recombinaison in vivo sans avoir recours à une sélection clonale. Ces méthodes, cellules et compositions sont utilisées dans l'automatisation de la recombinaison. Cette invention a aussi pour objet des méthodes de réalisation d'étapes séquentielles dans l'ingénierie génomique, ainsi que d'obtention d'une ingénierie génomique parallèle et multiplexe.

International search report:
Received at International Bureau: 23 October 2006 (23.10.2006) [US]

International Report on Patentability (IPRP) Chapter II of the PCT:
Not available

(81) Designated States:
AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
European Patent Office (EPO) : AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR
African Intellectual Property Organization (OAPI) : BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG
African Regional Intellectual Property Organization (ARIPO) : BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW
Eurasian Patent Organization (EAPO) : AM, AZ, BY, KG, KZ, MD, RU, TJ, TM