(12) International Application Status Report

Received at International Bureau: 28 January 2005 (28.01.2005)

Information valid as of: Not available

Report generated on: 09 December 2019 (09.12.2019)

(10) Publication number: (43) Publication date: (26) Publication language:
WO 2005/06172607 July 2005 (07.07.2005) German (DE)

(21) Application number: (22) Filing date: (25) Filing language:
PCT/DE2004/00272313 December 2004 (13.12.2004) German (DE)

(31) Priority number(s): (32) Priority date(s): (33) Priority status:
103 61 489.3 (DE)23 December 2003 (23.12.2003) Priority document received (in compliance with PCT Rule 17.1)

(51) International Patent Classification:
C12Q 1/68 (2006.01)

(71) Applicant(s):
JUSTUS-LIEBIG-UNIVERSITÄT GIESSEN [DE/DE]; Ludwigstrasse 23 35390 Giessen (DE) (for all designated states except US)
BÜLTE, Michael [DE/DE]; Brückenstrasse 12 35305 Reinhardshain (DE) (for US only)
SCHÖNENBRÜCHER, Holger [DE/DE]; Bitzenstrasse 34 35398 Giessen (DE) (for US only)
ABDULMAWJOOD, Amir [DE/DE]; Carl-Ulrich-Strasse 12 35396 Giessen (DE) (for US only)

(72) Inventor(s):
BÜLTE, Michael; Brückenstrasse 12 35305 Reinhardshain (DE)
SCHÖNENBRÜCHER, Holger; Bitzenstrasse 34 35398 Giessen (DE)
ABDULMAWJOOD, Amir; Carl-Ulrich-Strasse 12 35396 Giessen (DE)

(74) Common Representative(s):
TRANSMIT GESSELLSCHAFT FÜR TECHNOLOGIETRANSFER MBH; Kerkrader Strasse 3 35394 Giessen (DE)

(54) Title (EN): ANIMAL SPECIES-SPECIFIC AND QUANTITATIVE DETECTION OF CENTRAL NERVOUS SYSTEM TISSUE IN MEAT AND MEAT PRODUCTS
(54) Title (FR): DETECTION QUANTITATIVE ET SPECIFIQUE D'UNE ESPECE ANIMALE DE TISSUS DU SYSTEME NERVEUX CENTRAL DANS DE LA VIANDE ET DES PRODUITS CARNES
(54) Title (DE): TIERARTSPEZIFISCHER UND QUANTITATIVER NACHWEIS VON ZNS-GEWEBE IN FLEISCH UND FLEISCHERZEUGNISSEN

(57) Abstract:
(EN): The invention relates to a method for carrying out the animal species-specific and quantitative detection of central nervous system tissue in meat and meat products of ruminant animal species: cattle, sheep, goats or pigs with a real-time PCR method while using the glial fibrillary acidic protein (GFAP) messenger (m)-RNA. The inventive method is very reliable and can carried out even in heat-treated samples.
(FR): Procédé de détection quantitative et spécifique d'une espèce animale de tissus du système nerveux central dans de la viande et des produits carnés des espèces de ruminants que sont le boeuf, le mouton et la chèvre ou de porc à l'aide d'un procédé de PCR en temps réel reposant sur l'utilisation d'ARNm de protéine acide des filaments intermédiaires gliaux (GFAP). Ledit procédé est très fiable et peut être appliqué de manière simple, même dans des échantillons traités à la chaleur.
(DE): Die vorliegende Erfindung betrifft ein Verfahren zum tierartspezifischen und quantitativen Nachweis von ZNS-Gewebe in Fleisch und Fleischerzeugnissen der Wiederkäuer-Tierarten Rind, Schaf und Ziege oder Schwein mit einem Real Time-PCR-Verfahren unter Verwendung von saurer Gliafaserprotein (GFAP) messenger (m)-RNA. Das Verfahren ist sehr sicher und einfach auch in Hitze behandelten Proben durchführbar.

International search report:
Received at International Bureau: 23 June 2005 (23.06.2005) [EP]

International Report on Patentability (IPRP) Chapter II of the PCT:
Not available

(81) Designated States:
AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
European Patent Office (EPO) : AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR
African Intellectual Property Organization (OAPI) : BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG
African Regional Intellectual Property Organization (ARIPO) : BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW
Eurasian Patent Organization (EAPO) : AM, AZ, BY, KG, KZ, MD, RU, TJ, TM